DNA Testing

Atomic force microscopy in food preservation research: New insights to overcome spoilage issues

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The next degree of food security is required due to the fast-growing human inhabitants together with the elevated consciousness of wholesome existence. Currently, a big proportion of food is spoiled throughout storage and processing due to enzymes and microbial exercise, inflicting large financial losses to each producers and shoppers. Atomic force microscopy (AFM), as a robust scanning probe microscopy, has been efficiently and broadly used in food preservation analysis. This approach permits a non-invasive examination of food merchandise, offering high-resolution photos of floor construction and particular person polymers in addition to the bodily properties and adhesion of single molecules. In this paper, detailed purposes of AFM in food preservation are reviewed.

AFM has been used to present complete info in food preservation by evaluating the spoilage with its associated construction modification. By using AFM imaging and force measurement operate, the principle mechanisms concerned in the lack of food high quality and preservation applied sciences improvement might be additional elucidated. It can also be able to exploring the actions of enzymes and microbes in influencing the standard of food merchandise throughout storage. AFM supplies complete options to overcome spoilage issues with its versatile capabilities and high-throughput outcomes. Further analysis and improvement of this novel approach in order to resolve built-in issues in food preservation are obligatory.

Simultaneous imaging of complementary absorption and fluorescence contrasts with excessive spatial decision is beneficial for biomedical research. However, standard dual-modal photoacoustic (PA) and fluorescence imaging methods require using acoustic coupling media due to the contact operation of PA imaging, which causes issues and complicates the process in sure purposes corresponding to cell imaging and ophthalmic imaging. We current a novel dual-modal imaging system which mixes non-contact PA microscopy (PAM) based mostly on PA distant sensing and fluorescence microscopy (FLM) into one platform.

Assessment of the pressure depth sensitivity of Moiré sampling Scanning Transmission Electron Microscopy Geometrical Phase Analysis via a comparability with Dark-Field Electron Holography

In this examine, the Moiré sampling Scanning Transmission Electron Microscopy Geometrical Phase Analysis (or STEM Moiré GPA) pressure characterization methodology is in contrast to the well-established Dark-Field Electron Holography approach on a skinny movie stack grown by Molecular Beam Epitaxy. While experimental knowledge obtained with the 2 methods are, total, in good qualitative settlement, small statistically related variations are domestically noticed between the 2 strategies.

The outcomes obtained from each methods are additional confronted with Finite Element Method (FEM) mechanical simulations modeling the pressure rest phenomena from a skinny lamella. The FEM simulation highlights a non-uniform deformation area alongside the beam propagation path with a better deformation degree close to the floor of the lamella in contrast to the middle of the identical lamella. The system permits excessive lateral decision of two and a couple of.7 µm for PAM and FLM modes, respectively. In vivo imaging of a zebrafish larva injected with a rhodamine B answer is demonstrated, with PAM visualizing the pigment and FLM revealing the injected rhodamine B.

The center-surface pressure variations obtained from modeling are per the experimentally derived variations accounting for the truth that the SMG methodology is delicate to the pressure state of the floor of the lamella with a really slim depth-of-field, and the DFEH approach is measuring the pressure state of the middle of the identical lamella averaging over a big part of the thickness. The depth-of-field distinction between each strategies might be fairly associated to their respective distinction mechanisms (STEM vs Conventional Transmission Electron Microscopy). As the SMG methodology is utilizing a convergent probe, the slim depth-of-field could be used to sense the deformation area over completely different sections of the lamella utilizing the defocus and probably retrieve the three-dimensional pressure area.

Atomic force microscopy in food preservation research: New insights to overcome spoilage issues

Visualization of probiotics by way of epifluorescence microscopy and fluorescence in situ hybridization (FISH)

Aerobic plate counts, the usual for bacterial enumeration in the probiotic trade, might be biased in the direction of fast-growing organisms that replicate on artificial media and might considerably underestimate complete bacterial abundance. Culture-independent approaches corresponding to fluorescence in situ hybridization (FISH) maintain promise as a way to quickly and precisely enumerate micro organism in probiotic merchandise. In addition, FISH has the potential to extra precisely signify bacterial progress dynamics in the atmosphere in which merchandise are utilized with out imposing extra progress constraints which are required for enumeration by way of plate counts.
In this examine, we designed and optimized three new FISH probes to visualize and quantify Bacillus amyloliquefaciens, Bacillus pumilus, and Bacillus licheniformis inside probiotic merchandise. Microscopy-based estimates have been constant or greater than label claims for Pediococcus acidilactici, Pediococcus pentosaceus, Lactobacillus plantarum, Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus pumilus in each a direct fed microbial (DFM) product in addition to a crop microbial biostimulant (CMB) product. Quantification with FISH after a germination experiment revealed the potential for this method to be used after utility of the product

Peanut oil
GK9829-1L 1 l
EUR 130

Peanut oil
GK9829-250ML 250 ml
EUR 70

Peanut oil
GK9829-500ML 500 ml
EUR 90

Jojoba oil
GL1962-100ML 100 ml
EUR 78

Jojoba oil
GL1962-500ML 500 ml
EUR 190

Olive oil
GL2132-100ML 100 ml
EUR 46

Olive oil
GL2132-500ML 500 ml
EUR 66

Bergamot oil
GL2537-100ML 100 ml
EUR 70

Bergamot oil
GL2537-1L 1 l
EUR 229

Bergamot oil
GL2537-500ML 500 ml
EUR 150

Cottonseed oil
GL3259-1L 1 l
EUR 70

Cottonseed oil
GL3259-500ML 500 ml
EUR 53

Soybean oil
GL3290-1L 1 l
EUR 98

Soybean oil
GL3290-250ML 250 ml
EUR 59

Borage oil
GL5712-100G 100 g
EUR 154

Borage oil
GL5712-50G 50 g
EUR 102

Cedar oil
GL6882-25ML 25 ml
EUR 46

Cedar oil
GL6882-500ML 500 ml
EUR 150

Corn oil
GL7753-500ML 500 ml
EUR 58

Sesame oil
GL9605-1L 1 l
EUR 102

Sesame oil
GL9605-250ML 250 ml
EUR 62

Clove oil
GK8390-100ML 100 ml
EUR 70

Cedar oil
GL6882-100ML 100 ml
EUR 70

Immersion oil
GW3483-100ML 100 ml
EUR 58

Mineral oil
GX1229-1L 1 l
EUR 48

Mineral oil
GX1229-250ML 250 ml
EUR 39

Mineral oil
GX1229-500ML 500 ml
EUR 43

Mineral oil
GX1229-5L 5 l
EUR 95

Paraffin oil (Mineral oil), extra heavy fraction
PC5532 100ml
EUR 58.7

Oil Red O Stain Kit (For Fat)
ORK-1 1 kit(s)
EUR 192

Oil Red O Stain Kit (For Fat)
ORK-2 100 Slides
EUR 122

Oil Red O
OD0395 50g
EUR 65.66

DL-Methioninol (oil)
5-07713 25g Ask for price

DL-Methioninol (oil)
5-07714 100g Ask for price

Valerian root oil
20-abx186597
  • EUR 286.00
  • EUR 230.00
  • 1 kg
  • 500 g

Sunflower seed oil
GK2233-250ML 250 ml
EUR 69

Tea tree oil
GK8936-100ML 100 ml
EUR 53

Tea tree oil
GK8936-1KG 1 kg
EUR 138

Sunflower seed oil
GK2233-100ML 100 ml
EUR 49

Silicone oil, 500 cSt
GK1916-1L 1 l
EUR 150

Silicone oil, 500 cSt
GK1916-250ML 250 ml
EUR 78

Ethoxylated hydrogenated castor oil
GK2628-200G 200 g
EUR 166

Silicone oil, 350 cSt
GK4111-100ML 100 ml
EUR 46

Silicone oil, 350 cSt
GK4111-1L 1 l
EUR 94

Silicone oil, 350 cSt
GK4111-500ML 500 ml
EUR 70

Cinnamon oil, from leaf
GL3074-100G 100 g
EUR 78

Cinnamon oil, from leaf
GL3074-25G 25 g
EUR 52

Castor oil, EP grade
GL4383-1L 1 l
EUR 56

Castor oil, EP grade
GL4383-500ML 500 ml
EUR 46

Ethoxylated hydrogenated castor oil
GK2628-1KG 1 kg
EUR 269

Z-Leu-OH (oil)
C-2100.0025 25.0g
EUR 113
Description: Sum Formula: C14H19NO4; CAS# [2018-66-8]

Z-Leu-OH (oil)
C-2100.0100 100.0g
EUR 171
Description: Sum Formula: C14H19NO4; CAS# [2018-66-8]

Oil Red O Solution
ORG125 125 ml
EUR 76

Oil Red O Solution
ORG500 500 ml
EUR 140

Oil Red O Solution
ORG999 1000 ml
EUR 216

Xylene - Peanut Oil Solution
XPO125 125 ml
EUR 72

Xylene - Peanut Oil Solution
XPO500 500 ml
EUR 104

Xylene - Peanut Oil Solution
XPO999 1000 ml
EUR 136

H-Thr-Ome(Oil)
A6739-100000 100 g
EUR 316
Description: H-Thr-Ome(Oil)

H-Thr-Ome(Oil)
A6739-25000 25 g
EUR 183
Description: H-Thr-Ome(Oil)

Peanut Oil vaccine adjuvant
AV-5010-50 50 ml
EUR 225

Mineral Oil vaccine adjuvant
AV-5020-50 50 ml
EUR 225

Vacuum pump, oil free
FB-P-033A 1set
EUR 432.75
Description: Vacuum pump, oil free

Fine natural hair brush for mounting sections
PO501 each
EUR 67

Capsaicin, natural
GP1219-1G 1 g
EUR 91

Capsaicin, natural
GP1219-250MG 250 mg
EUR 59

Capsaicin, natural
GP1219-5G 5 g
EUR 245

Resveratrol, natural
GP2549-100MG 100 mg
EUR 62

Resveratrol, natural
GP2549-1G 1 g
EUR 166

Resveratrol, natural
GP2549-250MG 250 mg
EUR 94

Lactacystin (Natural)
2434-200
EUR 316

DiI in vegetable oil: (0.5mL)
60018 0.5ML
EUR 204
Description: Minimum order quantity: 1 unit of 0.5ML

Neuro-dio in vegetable oil
60019 0.2ML
EUR 259
Description: Minimum order quantity: 1 unit of 0.2ML

Neuro-DiI in vegetable oil
60020 0.2ML
EUR 259
Description: Minimum order quantity: 1 unit of 0.2ML

Z-D-Leu-OH (oil)
C-2105.0005 5.0g
EUR 113
Description: Sum Formula: C14H19NO4; CAS# [28862-79-5]

Z-D-Leu-OH (oil)
C-2105.0025 25.0g
EUR 335
Description: Sum Formula: C14H19NO4; CAS# [28862-79-5]

Z-D-Leu-OH (oil)
C-2105.0100 100.0g
EUR 959
Description: Sum Formula: C14H19NO4; CAS# [28862-79-5]

Oil Red O (C.I. 26125)
GT6537-100G 100 g
EUR 70

Oil Red O (C.I. 26125)
GT6537-25G 25 g
EUR 44

100-1000ul Natural Tip, Natural, Sterile, 100/Rack
BT222-RS 1PK, 100UNIT
EUR 54.94

(+)-Abscisic Acid (natural)
A153-100MG 100 mg
EUR 119

(+)-Abscisic Acid (natural)
A153-1G 1 g
EUR 366

(+)-Abscisic Acid (natural)
A153-250MG 250 mg
EUR 164

Boc-N-Me-Nle-OH (oil)
5-05519 5g Ask for price

Boc-N-Me-Nle-OH (oil)
5-05520 25g Ask for price

Squalene (oil-in-water nano emulsion)
VAdv-Ly0061 10 mL
EUR 372
Description: Squalene, a squalene-based oil-in-water nanoemulsion vaccine adjuvant.

Olive oil, extra virgin, EP grade
GL3812-100ML 100 ml
EUR 45

Olive oil, extra virgin, EP grade
GL3812-1L 1 l
EUR 80

Olive oil, extra virgin, EP grade
GL3812-500ML 500 ml
EUR 59

Paraffin oil, BP, Ph. Eur. grade
GL4141-1L 1 l
EUR 58

Paraffin oil, BP, Ph. Eur. grade
GL4141-2500ML 2500 ml
EUR 86

Boc-Lys(Z)(isopropyl)-OH (oil)
A-2890.0001 1.0g
EUR 345
Description: Sum Formula: C22H34N2O6; CAS# [125323-99-1]
How nanoparticles distribute in dwelling cells and overcome mobile boundaries are necessary standards in the design of drug carriers. Pair-correlation microscopy is a correlation evaluation of fluctuation in the fluorescence depth obtained by a confocal line scan that may quantify the dynamic properties of nanoparticle diffusion together with the variety of cell nanoparticles, diffusion coefficient, and transit time throughout a spatial distance. Due to the potential heterogeneities in nanoparticle properties and the complexity inside the mobile atmosphere, quantification of averaged auto- and pair-correlation profiles could obscure necessary insights into the flexibility of nanoparticles to ship medication.

Deep learning for the classification of medical kidney disease: a pilot study for electron microscopy

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Deep learning for the classification of medical kidney disease: a pilot study for electron microscopy

Artificial intelligence (AI) is a new frontier and infrequently enigmatic for medical professionals. Cloud computing might open up the area of laptop imaginative and prescient to a wider medical viewers and deep learning on the cloud permits one to design, develop, practice and deploy purposes with ease. In the area of histopathology, the implementation of numerous purposes in AI has been profitable for entire slide pictures wealthy in organic range. However, the evaluation of different tissue medias, together with electron microscopy, is but to be explored.

The current study goals to guage deep learning for the classification of medical kidney illness on electron microscopy pictures: amyloidosis, diabetic glomerulosclerosis, membranous nephropathy, membranoproliferative glomerulonephritis (MPGN), and skinny basement membrane illness (TBMD). We discovered good total classification with the MedKidneyEM-v1 Classifier and when regular and diseased kidneys, the common space underneath the curve for precision and recall was 0.841. The common space underneath the curve for precision and recall on the illness solely cohort was 0.909.

Digital pathology will form a new period for medical kidney illness and the current study demonstrates the feasibility of deep learning for electron microscopy. Future approaches could possibly be utilized by renal pathologists to enhance diagnostic concordance, decide therapeutic methods, and optimize affected person outcomes in a true medical surroundings. Without refined information inversion algorithms, nonlinear optical microscopy can purchase pictures at subcellular decision and comparatively massive depth, with believable endogenous contrasts indicative of genuine organic and pathological states.

We report a new method to visualise the native distribution of molecular recognition websites with nanoscale decision by amplitude-modulation atomic power microscopy. By integrating chemical modification of probes, photothermal excitation to drive a cantilever, and lift-up scanning over floor topography, we efficiently visualized binding websites supplied by streptavidin on a stable floor for biotin connected on an AFM probe. The optimization of measurement situations was mentioned intimately, and the software of the approach was verified with a totally different ligand-receptor system.

Modular Imaging Scaffold for Single-Particle Electron Microscopy

Technological breakthroughs in electron microscopy (EM) have made it attainable to unravel constructions of organic macromolecular complexes and to lift novel challenges, particularly associated to pattern preparation and heterogeneous macromolecular assemblies resembling DNA-protein, protein-protein, and membrane protein assemblies. Here, we constructed a V-shaped DNA origami as a scaffolding molecular system to template proteins at user-defined positions in area. This template positions macromolecular assemblies of numerous sizes, juxtaposes mixtures of biomolecules into advanced preparations, isolates biomolecules of their energetic state, and stabilizes membrane proteins in answer.

In addition, the design may be engineered to tune DNA mechanical properties by exerting a managed piconewton (pN) power on the molecular system and thus tailored to characterize mechanosensitive proteins. The binding website can be particularly personalized to accommodate the protein of curiosity, both interacting spontaneously with DNA or by directed chemical conjugation, rising the vary of potential targets for single-particle EM investigation. We assessed the applicability for 5 totally different proteins. Finally, as a proof of precept, we used RNAP protein to validate the method and to discover the compatibility of the template with cryo-EM pattern preparation.

Although impartial contrasts have been derived by sequentially imaging the similar pattern aircraft or quantity underneath totally different and infrequently optimized excitation situations, new laser supply engineering with inputs from key biomolecules surprisingly allow real-time simultaneous acquisition of a number of endogenous molecular contrasts to section a wealthy set of mobile and extracellular parts. Since this improvement permits easy single-beam single-shot excitation and simultaneous multicontrast epidirected sign detection, the ensuing platform avoids perturbative pattern pretreatments resembling fluorescent labeling, mechanical sectioning, scarce or interdependent distinction technology, constraints to the pattern or imaging geometry, and intraimaging movement artifacts which have restricted in vivo nonlinear optical molecular imaging.

Deep learning for the classification of medical kidney disease: a pilot study for electron microscopy

Sensing morphogenesis of bone cells underneath microfluidic shear stress by holographic microscopy and automated aberration compensation with deep learning

We current sensing time-lapse morphogenesis of residing bone cells underneath micro-fluidic shear stress (FSS) by digital holographic (DH) microscopy. To take away the impact of aberrations on quantitative measurements, we suggest a numerical and automated technique to compensate for aberrations primarily based on a convolutional neural community (CNN). For the first time, the aberration compensation situation is taken into account as a regression process the place optimum coefficients for establishing the part aberration map act as responses akin to the enter aberrated part picture.

DNASE & TOLUIDINE BLUE
D04-103-2Kg 2 Kg
EUR 2093

DNASE & TOLUIDINE BLUE
D04-103-500g 500 g
EUR 605

Toluidine Blue (C.I. 50240)
GT7960-100G 100 g
EUR 150

Toluidine Blue (C.I. 50240)
GT7960-25G 25 g
EUR 70

Toluidine Blue (C.I. 50240)
GT7960-5G 5 g
EUR 46

OPTIONAL BLUE FILTER (497NM)
GDBL-BLUE 1/pk
EUR 1082
Description: Lab Equipment; Axygen Branded EQ

conversion screen uv/blue
VLFC26-BLUE ea
EUR 606

N,N-Diethyl-o-toluidine
20-abx185990
  • EUR 551.00
  • EUR 272.00
  • 250 g
  • 50 g

2-O-Benzyl-3-O-allyl-sn-glycerol
O-1005.0001 1.0g
EUR 225
Description: Sum Formula: C13H18O3; CAS# [106401-57-4]

2-O-Benzyl-3-O-allyl-sn-glycerol
O-1005.0005 5.0g
EUR 803
Description: Sum Formula: C13H18O3; CAS# [106401-57-4]

1-O-Hexadecyl-2-O-methyl-sn-glycerol
O-1245.0250 250.0mg
EUR 151
Description: Sum Formula: C20H42O3; CAS# [96960-92-8]

1-O-Hexadecyl-2-O-methyl-sn-glycerol
O-1245.1000 1.0g
EUR 393
Description: Sum Formula: C20H42O3; CAS# [96960-92-8]

1-O-Hexadecyl-2-O-methyl-rac-glycerol
O-1350.0250 250.0mg
EUR 151
Description: Sum Formula: C20H42O3; CAS# [84337-41-7]

1-O-Hexadecyl-2-O-methyl-rac-glycerol
O-1350.1000 1.0g
EUR 393
Description: Sum Formula: C20H42O3; CAS# [84337-41-7]

1-O-Octadecyl-2-O-benzyl-sn-glycerol
O-1360.0001 1.0g
EUR 126
Description: Sum Formula: C28H50O3; CAS# [80707-93-3]

1-O-Octadecyl-2-O-benzyl-sn-glycerol
O-1360.0005 5.0g
EUR 418
Description: Sum Formula: C28H50O3; CAS# [80707-93-3]

3-O-Hexadecyl-2-O-acetyl-sn-glycero-1-phosphocholine
O-1255.0005 5.0mg
EUR 334
Description: Sum Formula: C26H54NO7P; CAS# [117985-57-6]

3-O-Hexadecyl-2-O-acetyl-sn-glycero-1-phosphocholine
O-1255.0025 25.0mg
EUR 1240
Description: Sum Formula: C26H54NO7P; CAS# [117985-57-6]

1-O-Hexadecyl-2-O-acetyl-sn-glycero-3-phosphocholine
O-1270.0025 25.0mg
EUR 212
Description: Sum Formula: C26H54NO7P; CAS# [74389-68-7]

1-O-Hexadecyl-2-O-acetyl-sn-glycero-3-phosphocholine
O-1270.0100 100.0mg
EUR 515
Description: Sum Formula: C26H54NO7P; CAS# [74389-68-7]

1-O-Hexadecyl-2-O-acetyl-sn-glycero-3-phosphocholine
O-1270.0500 500.0mg
EUR 1844
Description: Sum Formula: C26H54NO7P; CAS# [74389-68-7]

1-O-Octadecyl-2-O-acetyl-sn-glycero-3-phosphocholine
O-1355.0025 25.0mg
EUR 212
Description: Sum Formula: C28H58NO7P; CAS# [74389-69-8]

1-O-Octadecyl-2-O-acetyl-sn-glycero-3-phosphocholine
O-1355.0100 100.0mg
EUR 515
Description: Sum Formula: C28H58NO7P; CAS# [74389-69-8]

1-O-Octadecyl-2-O-acetyl-sn-glycero-3-phosphocholine
O-1355.0500 500.0mg
EUR 1844
Description: Sum Formula: C28H58NO7P; CAS# [74389-69-8]

1-O-Octadecyl-2-O-methyl-sn-glycero-3-phosphocholine
O-1385.0025 25.0mg
EUR 273
Description: Sum Formula: C27H58NO6P; CAS# [77286-66-9]

1-O-Octadecyl-2-O-methyl-sn-glycero-3-phosphocholine
O-1385.0100 100.0mg
EUR 756
Description: Sum Formula: C27H58NO6P; CAS# [77286-66-9]

1-O-Octadecyl-2-O-methyl-rac-glycero-3-phosphocholine
O-1415.0025 25.0mg
EUR 225
Description: Sum Formula: C27H58NO6P; CAS# [70641-51-9]

1-O-Octadecyl-2-O-methyl-rac-glycero-3-phosphocholine
O-1415.0100 100.0mg
EUR 612
Description: Sum Formula: C27H58NO6P; CAS# [70641-51-9]

p-Toluidine
20-abx185551
  • EUR 189.00
  • EUR 272.00
  • EUR 230.00
  • 100 g
  • 1 kg
  • 500 g

1-O-(cis-9-Octadecenyl)-2-O-acetyl-sn-glycero-3-phosphocholine
O-1030.0050 50.0mg
EUR 393
Description: Sum Formula: C28H56NO7P; CAS# [85966-90-1]

1-O-(cis-9-Octadecenyl)-2-O-acetyl-sn-glycero-3-phosphocholine
O-1030.0250 250.0mg
EUR 1482
Description: Sum Formula: C28H56NO7P; CAS# [85966-90-1]

3-O-Allyl-sn-glycerol
O-1000.0001 1.0g
EUR 126
Description: Sum Formula: C6H12O3; CAS# [55941-86-1]

3-O-Allyl-sn-glycerol
O-1000.0005 5.0g
EUR 418
Description: Sum Formula: C6H12O3; CAS# [55941-86-1]

1-O-Benzyl-sn-glycerol
O-1010.0001 1.0g
EUR 273
Description: Sum Formula: C10H14O3; CAS# [17325-85-8]

1-O-Benzyl-sn-glycerol
O-1010.0005 5.0g
EUR 998
Description: Sum Formula: C10H14O3; CAS# [17325-85-8]

3-O-Benzyl-sn-glycerol
O-1015.0001 1.0g
EUR 212
Description: Sum Formula: C10H14O3; CAS# [56552-80-8]

3-O-Benzyl-sn-glycerol
O-1015.0005 5.0g
EUR 756
Description: Sum Formula: C10H14O3; CAS# [56552-80-8]

1,2-O-Dihexadecyl-sn-glycerol
O-1040.0001 1.0g
EUR 176
Description: Sum Formula: C35H72O3; CAS# [67337-03-5]

1,2-O-Dihexadecyl-sn-glycerol
O-1040.0005 5.0g
EUR 611
Description: Sum Formula: C35H72O3; CAS# [67337-03-5]

1,2-O-Dihexadecyl-rac-glycerol
O-1045.0001 1.0g
EUR 176
Description: Sum Formula: C35H72O3; CAS# [13071-60-8]

1,2-O-Dihexadecyl-rac-glycerol
O-1045.0005 5.0g
EUR 611
Description: Sum Formula: C35H72O3; CAS# [13071-60-8]

1,2-O-Dioctadecyl-sn-glycerol
O-1155.0001 1.0g
EUR 273
Description: Sum Formula: C39H80O3; CAS# [82188-61-2]

1,2-O-Dioctadecyl-sn-glycerol
O-1155.0005 5.0g
EUR 998
Description: Sum Formula: C39H80O3; CAS# [82188-61-2]

1,2-O-Dioctadecyl-rac-glycerol
O-1160.0001 1.0g
EUR 176
Description: Sum Formula: C39H80O3; CAS# [6076-38-6]

1,2-O-Dioctadecyl-rac-glycerol
O-1160.0005 5.0g
EUR 611
Description: Sum Formula: C39H80O3; CAS# [6076-38-6]

1-O-Hexadecyl-sn-glycerol
O-1285.0001 1.0g
EUR 225
Description: Sum Formula: C19H40O3; CAS# [506-03-6]

1-O-Hexadecyl-sn-glycerol
O-1285.0005 5.0g
EUR 756
Description: Sum Formula: C19H40O3; CAS# [506-03-6]

1-O-Hexadecyl-rac-glycerol
O-1290.0001 1.0g
EUR 126
Description: Sum Formula: C19H40O3; CAS# [6145-69-3]

1-O-Hexadecyl-rac-glycerol
O-1290.0005 5.0g
EUR 418
Description: Sum Formula: C19H40O3; CAS# [6145-69-3]

3-O-Hexadecyl-sn-glycerol
O-1295.0001 1.0g
EUR 176
Description: Sum Formula: C19H40O3; CAS# [10550-58-0]

3-O-Hexadecyl-sn-glycerol
O-1295.0005 5.0g
EUR 611
Description: Sum Formula: C19H40O3; CAS# [10550-58-0]

1-O-Octadecyl-sn-glycerol
O-1365.0001 1.0g
EUR 225
Description: Sum Formula: C21H44O3; CAS# [6129-13-1]

1-O-Octadecyl-sn-glycerol
O-1365.0005 5.0g
EUR 756
Description: Sum Formula: C21H44O3; CAS# [6129-13-1]

1-O-Octadecyl-rac-glycerol
O-1370.0005 5.0g
EUR 151
Description: Sum Formula: C21H44O3; CAS# [544-62-7]

1-O-Octadecyl-rac-glycerol
O-1370.0025 25.0g
EUR 515
Description: Sum Formula: C21H44O3; CAS# [544-62-7]

SCREW CAPS WITH "O" RINGS. BLUE
SCO-B 500/pk
EUR 370
Description: Micro Tubes; Screw-Cap Microtubes - Axygen

1,2-O-Dihexadecyl-sn-glycero-3-phosphocholine
O-1050.0250 250.0mg
EUR 151
Description: Sum Formula: C40H84NO6P; CAS# [36314-47-3]

1,2-O-Dihexadecyl-sn-glycero-3-phosphocholine
O-1050.1000 1.0g
EUR 393
Description: Sum Formula: C40H84NO6P; CAS# [36314-47-3]

1-O-Hexadecyl-sn-glycero-3-phosphocholine
O-1300.0025 25.0mg
EUR 212
Description: Sum Formula: C24H52NO6P; CAS# [52691-62-0]

1-O-Hexadecyl-sn-glycero-3-phosphocholine
O-1300.0100 100.0mg
EUR 515
Description: Sum Formula: C24H52NO6P; CAS# [52691-62-0]

1-O-Octadecyl-sn-glycero-3-phosphocholine
O-1380.0025 25.0mg
EUR 212
Description: Sum Formula: C26H56NO6P; CAS# [74430-89-0]

1-O-Octadecyl-sn-glycero-3-phosphocholine
O-1380.0100 100.0mg
EUR 515
Description: Sum Formula: C26H56NO6P; CAS# [74430-89-0]

1,2-O-Dihexadecyl-sn-glycero-3-phosphoethanolamine
O-1435.0250 250.0mg
EUR 212
Description: Sum Formula: C37H78NO6P; CAS# [61423-61-8]

1,2-O-Dihexadecyl-sn-glycero-3-phosphoethanolamine
O-1435.1000 1.0g
EUR 576
Description: Sum Formula: C37H78NO6P; CAS# [61423-61-8]

BCIP toluidine salt
BB0072 500mg
EUR 134.39

EZClick? O-GalNAc Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)
K560-100
EUR 490

EZClick? O-GlcNAc Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)
K714-100
EUR 512

1-O-(cis-9-Octadecenyl)-sn-glycero-3-phosphocholine
O-1035.0050 50.0mg
EUR 334
Description: Sum Formula: C26H54NO6P; CAS# [97802-55-6]

1-O-(cis-9-Octadecenyl)-sn-glycero-3-phosphocholine
O-1035.0250 250.0mg
EUR 1240
Description: Sum Formula: C26H54NO6P; CAS# [97802-55-6]

1-O-Hexadecyl-2-desoxy-2-amino-sn-glycerol
O-1520.0025 25.0mg
EUR 126
Description: Sum Formula: C19H41NO2; CAS# [136770-76-8]

1-O-Hexadecyl-2-desoxy-2-amino-sn-glycerol
O-1520.0100 100.0mg
EUR 321
Description: Sum Formula: C19H41NO2; CAS# [136770-76-8]

1-O-Hexadecyl-2-desoxy-2-amino-sn-glycerol
O-1520.0500 500.0mg
EUR 1118
Description: Sum Formula: C19H41NO2; CAS# [136770-76-8]

LOOPED SCREW CAPS WITH "O" RINGS. BLUE
SCO-LP-B 500/pk
EUR 376
Description: Micro Tubes; Screw-Cap Microtubes - Axygen

BCIP p-Toluidine Salt
abx082537-100mg 100 mg
EUR 203

Blue Base for Dissecting Board
M625 1 ea.
EUR 200

O' in 1 DNA Polymerase Premix 2xwi Blue Dye
FYT206-100P 100 Preps 1.25ml x 2 vials Ask for price

BCIP, toluidine (5-bromo-4-chloro-3-indoxyl phosphate, p-toluidine salt): (100mg)
10002 100MG
EUR 114
Description: Minimum order quantity: 1 unit of 100MG

BCIP, toluidine (5-bromo-4-chloro-3-indoxyl phosphate, p-toluidine salt): (500mg)
10002-1 500MG
EUR 288
Description: Minimum order quantity: 1 unit of 500MG

BCIP, toluidine (5-bromo-4-chloro-3-indoxyl phosphate, p-toluidine salt): (5g)
10002-2 5G
EUR 1241
Description: Minimum order quantity: 1 unit of 5G

ECOS Blue (XL1-Blue
FYE107-10VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue
FYE107-80VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue
FYE108-10VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue
FYE108-80VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue
FYE109-10VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue
FYE109-80VL 100 µl x 10 vials Ask for price

safePort? O-ring cap, HP, BLUE, sterile, 500 pcs/pk
502B-11pk
EUR 96

safePort? O-ring cap, LP, BLUE, sterile, 500 pcs/pk
505B-11pk
EUR 96

ORANGE LOOPED SCREW CAPS WITH "O" RINGS. FITS 0.5ML, 1.5ML AND 2.0ML TUBES
SCO-LP-O 500/pk
EUR 376
Description: Micro Tubes; Screw-Cap Microtubes - Axygen

safePort? O-ring loop cap, LP, BLUE, sterile, 500 pcs/pk
504B-11pk
EUR 98

Screw-Cap Microtubes, 1.5ml, sterile, O-ring, blue screw-caps attached
C3150-S 1000/pack
EUR 170.15

Screw-Cap Microtubes, 1.5ml, sterile, O-ring, blue loop-caps attached
C3150-SL 1000/pack
EUR 177.58

Monoclonal antibody for O-GlcNAc
SMC-502D 0.1mg
EUR 422
Description: A monoclonal antibody from clone 9H6 against Human O-GlcNAc. The host species for the production of this antibody is Mouse. The antigen used for immunization is Synthetic ?-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH).. The antibody is tested and validated for WB, ICC/IF, IHC, ELISA assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:50); ELISA (1:1000); IHC (1:25). This MAb for O-GlcNAc is not conjugated.

Monoclonal antibody for O-GlcNAc
SMC-502D-A390 0.1mg
EUR 469
Description: A monoclonal antibody from clone 9H6 against Human O-GlcNAc. The host species for the production of this antibody is Mouse. The antigen used for immunization is Synthetic ?-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH).. The antibody is tested and validated for WB, ICC/IF, IHC, ELISA assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:50); ELISA (1:1000); IHC (1:25). This MAb for O-GlcNAc is conjugated with ATTO 390.

Monoclonal antibody for O-GlcNAc
SMC-502D-A488 0.1mg
EUR 468
Description: A monoclonal antibody from clone 9H6 against Human O-GlcNAc. The host species for the production of this antibody is Mouse. The antigen used for immunization is Synthetic ?-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH).. The antibody is tested and validated for WB, ICC/IF, IHC, ELISA assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:50); ELISA (1:1000); IHC (1:25). This MAb for O-GlcNAc is conjugated with ATTO 488.

Monoclonal antibody for O-GlcNAc
SMC-502D-A565 0.1mg
EUR 468
Description: A monoclonal antibody from clone 9H6 against Human O-GlcNAc. The host species for the production of this antibody is Mouse. The antigen used for immunization is Synthetic ?-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH).. The antibody is tested and validated for WB, ICC/IF, IHC, ELISA assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:50); ELISA (1:1000); IHC (1:25). This MAb for O-GlcNAc is conjugated with ATTO 565.

Monoclonal antibody for O-GlcNAc
SMC-502D-A594 0.1mg
EUR 468
Description: A monoclonal antibody from clone 9H6 against Human O-GlcNAc. The host species for the production of this antibody is Mouse. The antigen used for immunization is Synthetic ?-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH).. The antibody is tested and validated for WB, ICC/IF, IHC, ELISA assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:50); ELISA (1:1000); IHC (1:25). This MAb for O-GlcNAc is conjugated with ATTO 594.

We adopted tens of hundreds of residing cells’ part pictures reconstructed from digital holograms for coaching the CNN. The experiments show that, primarily based on the educated community, part aberrations may be completely eliminated in real-time with none speculation of object and aberration part, data of the setup’s bodily parameters, and the operation of deciding on background areas; therefore, the morphogenesis of the bone cells underneath FSS is precisely detected and quantitatively analyzed. The outcomes present that the proposed technique might present a extremely environment friendly and versatile technique to examine the results of micro-FSS on residing organic cells in microfluidic lab-on-chip platforms because of the mixture of phase-contrast label-free microcopy with synthetic intelligence.

Non-invasive Quantification of Cell Wall Porosity by Fluorescence Quenching Microscopy

by
Non-invasive Quantification of Cell Wall Porosity by Fluorescence Quenching Microscopy

All micro organism, fungi and plant cells are surrounded by a cell wall. This complicated community of polysaccharides and glycoproteins gives mechanical assist, defines cell form, controls cell development and influences the trade of substances between the cell and its environment. Despite its identify, the cell wall is a versatile, dynamic construction. However, because of the lack of non-invasive strategies to probe the construction, comparatively little is understood concerning the synthesis and dynamic transforming of cell partitions. Here, we describe a non-invasive technique that quantifies a key physiological parameter of cell partitions, the porosity, i.e., the dimensions of areas between cell wall elements.

This technique measures the porosity-dependent lower of the plasma membrane-localized fluorescent dye FM4-64 within the presence of the extracellular quencher Trypan blue. This technique is utilized to micro organism, fungi and plant cell partitions to detect dynamic modifications of porosity in response to environmental cues.The most vital virulence issue within the Cryptococcus genus is the polysaccharide capsule. This genus contains a number of species that trigger life-threatening invasive illness. An enhance in capsule thickness is vital throughout fungal an infection.

The capsule is normally imaged utilizing India ink, and essential insights on the dynamics of its development have been obtained utilizing capsule-binding proteins reminiscent of particular antibodies or complement. We have developed another technique that permits each static and time-lapse imaging of the capsule utilizing Percoll®, a suspension of nanometric spheres that don’t penetrate the capsule. Given that these particles have a better refractive index than the capsule, the latter could be imaged by differential interference distinction (DIC) microscopy. Static commentary of the capsule with DIC and Percoll® ends in capsule thickness measurements that match these made with India ink.

Using capsule-inducing media, a glass-bottom incubation chamber and a live-imaging system outfitted for DIC microscopy, this technique permits time-lapse imaging of capsule development. In distinction with India ink staining, DIC imaging of Percoll® exclusion halos lead to crisp photos. The best benefit of this technique, although, is that in contrast to India ink, the Percoll® particles are non-toxic and in contrast to opsonins they don’t bind the capsule, leading to observations of capsule development which are free from interference of sure proteins on capsule physiology.

A microscopy-based strategy for figuring out development likelihood and lag time of particular person bacterial cells

The growth of related predictive fashions for single-cell lag time and development likelihood close to development limits is of important significance for predicting pathogen habits in meals. The classical strategies for information acquisition on this area are based mostly on turbidity measurements of tradition media in microplate wells inoculated with roughly one bacterial cell per effectively. Yet, these strategies are labour intensive and would profit from larger throughput. In this research, we developed a quantitative experimental technique utilizing automated microscopy to find out the single-cell development likelihood and lag time.
The developed technique consists of the use of direct cell commentary with phase-contrast microscopy outfitted with a 100× goal and a high-resolution gadget digicam. The technique isn’t a time-lapse technique however is predicated on the commentary of excessive numbers of colonies for a given time. Automation of picture acquisition and picture evaluation was used to achieve a excessive throughput. The single-cell development chances and lag occasions of 4 strains of Listeria monocytogenes have been decided at 4 °C. The microscopic technique was proven to be a promising technique for the dedication of particular person lag occasions and development likelihood on the single-cell stage.
We exhibit that beneath ambient and humidity-controlled situations, operation of bimodal excitation single-scan electrical drive microscopy with no electrical suggestions loop will increase the spatial decision of floor electrical property measurements all the way down to the 5 nm restrict. This technical enchancment is featured on epitaxial graphene layers on SiC, which is used as a mannequin pattern. The experimental situations developed to attain such decision are mentioned and linked to the secure imaging achieved utilizing the proposed technique.
The software of the herein reported technique is achieved with out the necessity to apply DC bias voltages, which advantages specimens which are extremely delicate to polarization. Besides, it permits the simultaneous parallel acquisition of floor electrical properties (reminiscent of contact potential distinction) on the similar scanning price as in amplitude modulation atomic drive microscopy (AFM) topography measurements. This makes it engaging for purposes in excessive scanning velocity AFM experiments in numerous fields for materials screening and metrology of semiconductor methods.
Non-invasive Quantification of Cell Wall Porosity by Fluorescence Quenching Microscopy

A bidirectional scanning technique for scanning transmission X-ray microscopy

Scanning mode is a key issue for the excellent efficiency, together with imaging effectivity, of scanning transmission X-ray microscopy (STXM). Herein is introduced a bidirectional scanning technique designed for STXM with an S-shaped transferring observe. In this technique, artificially designed ramp waves are generated by a piezo-stage controller to regulate the two-dimensional scanning of the pattern. The pattern place info is measured utilizing laser interferometric sensors and despatched to a field-programmable gate array (FPGA) board which additionally acquires the X-ray indicators concurrently from the detector.

Differentiating Solution (For Orcein Stain)
DSA125 125 ml
EUR 71

Differentiating Solution (For Orcein Stain)
DSA500 500 ml
EUR 122

Orcein Solution
OAA125 125 ml
EUR 66

Orcein Solution
OAA500 500 ml
EUR 109

Orcein Solution
OAA999 1000 ml
EUR 148

Orcein Stain Kit
K1432-125
EUR 501

Orcein Stain Kit
K1432-30
EUR 359

Orcein Stain Kit (For Hepatitis B and Elastic Fibers)
HBK-1 1 kit(s)
EUR 239

Orcein Stain Kit (For Hepatitis B and Elastic Fibers)
HBK-2 1 kit(s)
EUR 144

EZClick? Palmitoylated Protein Assay Kit (FACS/Microscopy), Red Fluorescence
K416-100
EUR 490

EZClick? Stearoylated Protein Assay Kit (FACS/Microscopy), Red Fluorescence
K418-100
EUR 490

EZClick? Palmitoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence
K452-100
EUR 490

EZClick? Stearoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence
K453-100
EUR 490

EZClick? Myristoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence
K497-100
EUR 490

Holder for Plasmid Midi, Maxi and Maxi plus, Ion Exchange column
FAPDE-holder-for-ion-exchange 1 prep
EUR 158

EZClick? Global Protein Synthesis Assay Kit (FACS/Microscopy), Green Fluorescence
K459-100
EUR 550

EZClick? Global RNA Synthesis Assay Kit (FACS/Microscopy), Red Fluorescence
K462-100
EUR 533

EZClick? Global Protein Synthesis Assay Kit (FACS/Microscopy), Red Fluorescence
K715-100
EUR 550

EZClick? Global Phospholipid Synthesis Assay Kit (FACS/Microscopy), Red Fluorescence
K717-100
EUR 512

EZClick? Global RNA Synthesis Assay Kit (FACS/Microscopy), Red Fluorescence
K718-100
EUR 533

EZClick? O-GalNAc Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)
K560-100
EUR 490

EZClickTM Fucose (FucAz) Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)
K592-100
EUR 490

EZClick? O-GlcNAc Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)
K714-100
EUR 512

EZClick? EdU Cell Proliferation/DNA Synthesis Kit (FACS/Microscopy), Red Fluorescence
K946-100
EUR 533

EZClick? Sialic Acid (ManAz) Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)
K441-100
EUR 490

Adaptor for flexible plates, accessory for AgileSealer?
APS-200-FP
EUR 351

Sample comb for batching for AE-6110
2398313 unit
EUR 100
Description: (90mm・r efer ence at t ached・1.5mm t i ck ness)

gelcaster for sequencer
ESEQ1000-CAST ea
EUR 437

Stand for D1000
BCM1237 1 pcs, 1 UNIT
EUR 556.7

For-Ala-OH
5-02335 25g Ask for price

For-Ala-OH
5-02336 100g Ask for price

For-Val-OH
5-03697 25g Ask for price

For-Val-OH
5-03698 100g Ask for price

Antibody for CD9
SPC-1272D 0.1ml
EUR 314
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is unconjugated.

Antibody for CD9
SPC-1272D-A390 0.1ml
EUR 361
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to ATTO 390.

Antibody for CD9
SPC-1272D-A488 0.1ml
EUR 360
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to ATTO 488.

Antibody for CD9
SPC-1272D-A565 0.1ml
EUR 360
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to ATTO 565.

Antibody for CD9
SPC-1272D-A594 0.1ml
EUR 360
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to ATTO 594.

Antibody for CD9
SPC-1272D-A633 0.1ml
EUR 360
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to ATTO 633.

Antibody for CD9
SPC-1272D-A655 0.1ml
EUR 360
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to ATTO 655.

Antibody for CD9
SPC-1272D-A680 0.1ml
EUR 360
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to ATTO 680.

Antibody for CD9
SPC-1272D-A700 0.1ml
EUR 360
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to ATTO 700.

Antibody for CD9
SPC-1272D-ALP 0.1ml
EUR 355
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to Alkaline Phosphatase.

Antibody for CD9
SPC-1272D-APC 0.1ml
EUR 359
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to APC .

Antibody for CD9
SPC-1272D-APCCY7 0.1ml
EUR 432
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to APC/Cy7.

Antibody for CD9
SPC-1272D-BI 0.1ml
EUR 357
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to Biotin.

Antibody for CD9
SPC-1272D-DY350 0.1ml
EUR 436
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to Dylight 350.

Antibody for CD9
SPC-1272D-DY405 0.1ml
EUR 412
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to Dylight 405.

Antibody for CD9
SPC-1272D-DY488 0.1ml
EUR 393
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to Dylight 488.

Antibody for CD9
SPC-1272D-DY594 0.1ml
EUR 397
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to Dylight 594.

Antibody for CD9
SPC-1272D-DY633 0.1ml
EUR 387
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to Dylight 633.

Antibody for CD9
SPC-1272D-FITC 0.1ml
EUR 353
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to FITC.

Antibody for CD9
SPC-1272D-HRP 0.1ml
EUR 349
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to HRP.

Antibody for CD9
SPC-1272D-P594 0.1ml
EUR 367
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to PE/ATTO 594.

Antibody for CD9
SPC-1272D-PCP 0.1ml
EUR 359
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to PerCP.

Antibody for CD9
SPC-1272D-RPE 0.1ml
EUR 358
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to RPE .

Antibody for CD9
SPC-1272D-STR 0.1ml
EUR 359
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to Streptavidin.

Antibody for KDEL
SPC-109A 0.05mg
EUR 205
Description: A polyclonal antibody for KDEL from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with KDEL containing peptide immunogen. The Antibody is tested and validated for WB, ICC/IF, IHC assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This KDEL antibody is unconjugated.

Antibody for KDEL
SPC-109B 0.2mg
EUR 350
Description: A polyclonal antibody for KDEL from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with KDEL containing peptide immunogen. The Antibody is tested and validated for WB, ICC/IF, IHC assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This KDEL antibody is unconjugated.

Antibody for KDEL
SPC-109B-A390 0.2mg
EUR 439
Description: A polyclonal antibody for KDEL from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with KDEL containing peptide immunogen. The Antibody is tested and validated for WB, ICC/IF, IHC assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This KDEL antibody is conjugated to ATTO 390.

Antibody for KDEL
SPC-109B-A488 0.2mg
EUR 436
Description: A polyclonal antibody for KDEL from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with KDEL containing peptide immunogen. The Antibody is tested and validated for WB, ICC/IF, IHC assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This KDEL antibody is conjugated to ATTO 488.

Antibody for KDEL
SPC-109B-A565 0.2mg
EUR 436
Description: A polyclonal antibody for KDEL from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with KDEL containing peptide immunogen. The Antibody is tested and validated for WB, ICC/IF, IHC assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This KDEL antibody is conjugated to ATTO 565.

Antibody for KDEL
SPC-109B-A594 0.2mg
EUR 436
Description: A polyclonal antibody for KDEL from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with KDEL containing peptide immunogen. The Antibody is tested and validated for WB, ICC/IF, IHC assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This KDEL antibody is conjugated to ATTO 594.

Antibody for KDEL
SPC-109B-A633 0.2mg
EUR 436
Description: A polyclonal antibody for KDEL from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with KDEL containing peptide immunogen. The Antibody is tested and validated for WB, ICC/IF, IHC assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This KDEL antibody is conjugated to ATTO 633.

Antibody for KDEL
SPC-109B-A655 0.2mg
EUR 436
Description: A polyclonal antibody for KDEL from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with KDEL containing peptide immunogen. The Antibody is tested and validated for WB, ICC/IF, IHC assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This KDEL antibody is conjugated to ATTO 655.

Antibody for KDEL
SPC-109B-A680 0.2mg
EUR 436
Description: A polyclonal antibody for KDEL from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with KDEL containing peptide immunogen. The Antibody is tested and validated for WB, ICC/IF, IHC assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This KDEL antibody is conjugated to ATTO 680.

Antibody for KDEL
SPC-109B-A700 0.2mg
EUR 436
Description: A polyclonal antibody for KDEL from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with KDEL containing peptide immunogen. The Antibody is tested and validated for WB, ICC/IF, IHC assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This KDEL antibody is conjugated to ATTO 700.

Antibody for KDEL
SPC-109B-ALP 0.2mg
EUR 425
Description: A polyclonal antibody for KDEL from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with KDEL containing peptide immunogen. The Antibody is tested and validated for WB, ICC/IF, IHC assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This KDEL antibody is conjugated to Alkaline Phosphatase.

Antibody for KDEL
SPC-109B-APC 0.2mg
EUR 435
Description: A polyclonal antibody for KDEL from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with KDEL containing peptide immunogen. The Antibody is tested and validated for WB, ICC/IF, IHC assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This KDEL antibody is conjugated to APC .

Antibody for KDEL
SPC-109B-APCCY7 0.2mg
EUR 585
Description: A polyclonal antibody for KDEL from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with KDEL containing peptide immunogen. The Antibody is tested and validated for WB, ICC/IF, IHC assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This KDEL antibody is conjugated to APC/Cy7.

Antibody for KDEL
SPC-109B-BI 0.2mg
EUR 427
Description: A polyclonal antibody for KDEL from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with KDEL containing peptide immunogen. The Antibody is tested and validated for WB, ICC/IF, IHC assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This KDEL antibody is conjugated to Biotin.

Antibody for KDEL
SPC-109B-DY350 0.2mg
EUR 471
Description: A polyclonal antibody for KDEL from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with KDEL containing peptide immunogen. The Antibody is tested and validated for WB, ICC/IF, IHC assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This KDEL antibody is conjugated to Dylight 350.

Antibody for KDEL
SPC-109B-DY405 0.2mg
EUR 448
Description: A polyclonal antibody for KDEL from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with KDEL containing peptide immunogen. The Antibody is tested and validated for WB, ICC/IF, IHC assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This KDEL antibody is conjugated to Dylight 405.

Antibody for KDEL
SPC-109B-DY488 0.2mg
EUR 428
Description: A polyclonal antibody for KDEL from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with KDEL containing peptide immunogen. The Antibody is tested and validated for WB, ICC/IF, IHC assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This KDEL antibody is conjugated to Dylight 488.

Antibody for KDEL
SPC-109B-DY594 0.2mg
EUR 432
Description: A polyclonal antibody for KDEL from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with KDEL containing peptide immunogen. The Antibody is tested and validated for WB, ICC/IF, IHC assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This KDEL antibody is conjugated to Dylight 594.

Antibody for KDEL
SPC-109B-DY633 0.2mg
EUR 422
Description: A polyclonal antibody for KDEL from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with KDEL containing peptide immunogen. The Antibody is tested and validated for WB, ICC/IF, IHC assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This KDEL antibody is conjugated to Dylight 633.

Antibody for KDEL
SPC-109B-FITC 0.2mg
EUR 420
Description: A polyclonal antibody for KDEL from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with KDEL containing peptide immunogen. The Antibody is tested and validated for WB, ICC/IF, IHC assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This KDEL antibody is conjugated to FITC.

Antibody for KDEL
SPC-109B-HRP 0.2mg
EUR 414
Description: A polyclonal antibody for KDEL from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with KDEL containing peptide immunogen. The Antibody is tested and validated for WB, ICC/IF, IHC assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This KDEL antibody is conjugated to HRP.

Antibody for KDEL
SPC-109B-P594 0.2mg
EUR 447
Description: A polyclonal antibody for KDEL from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with KDEL containing peptide immunogen. The Antibody is tested and validated for WB, ICC/IF, IHC assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This KDEL antibody is conjugated to PE/ATTO 594.

Antibody for KDEL
SPC-109B-PCP 0.2mg
EUR 435
Description: A polyclonal antibody for KDEL from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with KDEL containing peptide immunogen. The Antibody is tested and validated for WB, ICC/IF, IHC assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This KDEL antibody is conjugated to PerCP.

Antibody for KDEL
SPC-109B-RPE 0.2mg
EUR 429
Description: A polyclonal antibody for KDEL from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with KDEL containing peptide immunogen. The Antibody is tested and validated for WB, ICC/IF, IHC assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This KDEL antibody is conjugated to RPE .

Antibody for KDEL
SPC-109B-STR 0.2mg
EUR 432
Description: A polyclonal antibody for KDEL from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with KDEL containing peptide immunogen. The Antibody is tested and validated for WB, ICC/IF, IHC assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This KDEL antibody is conjugated to Streptavidin.

Antibody for KDEL
SPC-109S 0.012mg
EUR 65
Description: A polyclonal antibody for KDEL from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with KDEL containing peptide immunogen. The Antibody is tested and validated for WB, ICC/IF, IHC assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This KDEL antibody is unconjugated.

Antibody for Phosphoserine
SPC-149F 0.4ml
EUR 362
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is unconjugated.

Antibody for Phosphoserine
SPC-149F-A390 0.4ml
EUR 409
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to ATTO 390.

Antibody for Phosphoserine
SPC-149F-A488 0.4ml
EUR 408
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to ATTO 488.

Antibody for Phosphoserine
SPC-149F-A565 0.4ml
EUR 408
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to ATTO 565.

Antibody for Phosphoserine
SPC-149F-A594 0.4ml
EUR 408
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to ATTO 594.

Antibody for Phosphoserine
SPC-149F-A633 0.4ml
EUR 408
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to ATTO 633.

Antibody for Phosphoserine
SPC-149F-A655 0.4ml
EUR 408
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to ATTO 655.

Antibody for Phosphoserine
SPC-149F-A680 0.4ml
EUR 408
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to ATTO 680.

Antibody for Phosphoserine
SPC-149F-A700 0.4ml
EUR 408
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to ATTO 700.

Antibody for Phosphoserine
SPC-149F-ALP 0.4ml
EUR 403
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to Alkaline Phosphatase.

Antibody for Phosphoserine
SPC-149F-APC 0.4ml
EUR 408
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to APC .

Antibody for Phosphoserine
SPC-149F-APCCY7 0.4ml
EUR 480
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to APC/Cy7.

Antibody for Phosphoserine
SPC-149F-BI 0.4ml
EUR 405
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to Biotin.

Since the info recorded by the FPGA include the actual place of every scanned level, the affect of the backlash brought on by the back-turning motion on the STXM picture could be eradicated. By using an tailored post-processing program, a re-meshed high-resolution STXM picture could be obtained. This S-track bidirectional scanning technique in fly-scan mode has been carried out on the STXM endstation on the Shanghai Synchrotron Radiation Facility (SSRF), and efficiently resolved the ∼30 nm interval between the innermost strips of a Siemens star. This work removes the limitation on bidirectional scanning brought on by motor backlash and vibration, and considerably improves the effectivity of STXM experiments.

 

 

Electron Microscopy Sample Preparation Protocol Enabling Nano-to-mesoscopic Mapping of Cellular Connectomes and Their Habitats in Human Tissues and Organs

by
Electron Microscopy Sample Preparation Protocol Enabling Nano-to-mesoscopic Mapping of Cellular Connectomes and Their Habitats in Human Tissues and Organs

Multibeam scanning electron microscopy (multiSEM) gives a technical platform for seamless nano-to-mesoscale mapping of cells in human tissues and organs, which is a serious new initiative of the U.S. National Institutes of Health. Such cross-length-scale imaging is anticipated to supply unprecedented understanding of relationships between mobile well being and tissue-organ in addition to organismal-scale well being outcomes. For instance, understanding relationships between loss in cell viability and cell community connectivity allows identification of emergent behaviors and prediction of degenerative illness onset, in organs as numerous as bone and mind, at early timepoints, offering a foundation for future therapies and prevention.

Developed for fast throughput imaging of minute defects on semiconductor wafers, multiSEM has just lately been tailored for imaging of human organs, their constituent tissues, and their respective mobile inhabitants. Through integration of geospatial approaches, statistical and community modelling, advances in computing and the administration of immense datasets, in addition to current developments in machine studying that allow the automation of large knowledge analyses, multiSEM and different cross- reducing imaging applied sciences have the potential to exert a profound impression on elucidation of illness mechanisms, translating to enhancements in human well being. Here we offer a protocol for acquisition and preparation of pattern specimen sizes of diagnostic relevance for human anatomy and physiology.

Activation of sort 1 cannabinoid (CB1) receptors by endogenous, exogenous (hashish derivatives) or artificial cannabinoids (i.e., CP 55.940, Win-2) has all kinds of behavioral results because of the presence of CB1 receptors in the mind. In situ hybridization and immunohistochemical methods have been essential for outlining the CB1 receptor expression and localization on the mobile stage. Nevertheless, extra superior strategies are wanted to disclose the exact topography of CB1 receptors in the mind, particularly in unsuspected websites resembling different cell varieties and organelles with low receptor expression (e.g., glutamatergic neurons, astrocytes, mitochondria).

High-resolution immunoelectron microscopy gives a extra exact detection technique for the subcellular localization of CB1 receptors in the mind. Herein, we describe a single pre-embedding immunogold technique for electron microscopy primarily based on the use of particular CB1 receptor antibodies and silver-intensified 1.four nm gold-labeled Fab’ fragments, and a mixed pre-embedding immunogold and immunoperoxidase technique that employs biotinylated secondary antibodies and avidin-biotin-peroxidase complicated for the simultaneous localization of CB1 receptors and protein markers of particular mind cells or synapses (e.g., GFAP, GLAST, IBA-1, PSD-95, gephyrin).

Cryo-transmission Electron Microscopy of Outer-inner Membrane Vesicles Naturally Secreted by Gram-negative Pathogenic Bacteria

A protocol was developed to visualise and analyze the construction of membrane vesicles (MVs) from Gram-negative micro organism. It is now accepted that these micrometric spherical vesicles are generally produced by cells from all three domains of life, so the protocol may very well be helpful in the examine of vesicles produced by eukaryotes and archaea in addition to micro organism. The multiplicity of capabilities carried out by MVs, associated to cell communication, interplay with the immune system, pathogenesis, and nutrient acquisition, amongst others, has made MVs a sizzling matter of analysis. Due to their small measurement (25-300 nm), the statement of MVs requires electron microscopy and is often carried out by transmission electron microscopy (TEM) of negatively stained MVs.

Other protocols utilized for his or her visualization embrace scanning electron microscopy, TEM after fixation and embedding of vesicles, and even atomic power microscopy. In some of these methods, vesicle construction is altered by drying, whereas others are time-consuming and most of them can generate artifacts. Cryo-TEM after plunge freezing permits the visualization of samples embedded in a skinny movie of vitreous ice, which preserves their native mobile constructions and gives the very best accessible decision for the imaging. This is achieved by very excessive cooling charges that flip the intrinsic water of cells into vitreous ice, avoiding crystal formation and part segregation between water and solutes. In addition to different varieties of characterization, an correct information of MV construction, which could be obtained by this protocol, is important for MV utility in totally different fields.

Electron Microscopy Sample Preparation Protocol Enabling Nano-to-mesoscopic Mapping of Cellular Connectomes and Their Habitats in Human Tissues and Organs

Influence of age on sperm traits evaluated by mild and electron microscopies

The impression of male getting old on male fertility has solely just lately turn out to be of curiosity to the scientific group. This examine goals to evaluate the connection between age and fertility amongst a pattern of males, contemplating the person and pathological traits. In this retrospective examine knowledge of semen evaluation and medical historical past of 1294 Italian male sufferers had been thought-about.  A generalized linear mannequin was used to discover the affect of male age on semen high quality, contemplating as confounders wine consumption, smoking habits, presence of varicocele, consanguinity and optimistic semen bacteriological evaluation and urethral swab.

Rhodamine B [HRP]
DAGA-060H 1mg
EUR 1209

Rhodamine B [BSA]
DAGA-072B 1mg
EUR 780

Rhodamine B [KLH]
DAGA-078K 1mg
EUR 1079

Octadecyl rhodamine B
60033 10MG
EUR 281
Description: Minimum order quantity: 1 unit of 10MG

Rhodamine B base
GT5360-100G 100 g
EUR 158

Rhodamine B base
GT5360-10G 10 g
EUR 50

Rhodamine B base
GT5360-25G 25 g
EUR 70

Rhodamine B base
GT5360-50G 50 g
EUR 102

Rhodamine B ELISA Kit
DEIANJ26 96T
EUR 866
Description: The Rhodamine B ELISA Kit is an immunoassay for the quantitative and sensitive screening of Rhodamine B in food samples such as chili sauce and chili powder, etc.

Rhodamine B (RDB) Antibody
20-abx179008
  • EUR 439.00
  • EUR 133.00
  • EUR 1274.00
  • EUR 606.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rhodamine B (C.I. 45170)
GT7006-100G 100 g
EUR 62

Rhodamine B (C.I. 45170)
GT7006-250G 250 g
EUR 94

Rhodamine B (C.I. 45170)
GT7006-25G 25 g
EUR 46

Rhodamine B (RDB) Protein (OVA)
20-abx651895
  • EUR 578.00
  • EUR 258.00
  • EUR 1720.00
  • EUR 690.00
  • EUR 425.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

OVA Conjugated Rhodamine B (RDB)
4-CPO062Ge21
  • EUR 413.60
  • EUR 214.00
  • EUR 1276.00
  • EUR 492.00
  • EUR 884.00
  • EUR 340.00
  • EUR 3040.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Pan-species Rhodamine B expressed in: E.coli

Rhodamine B, hexyl ester, perchlorate
22211 10 mg
EUR 115

Rhodamine B solution, 0.2% in isopropanol
GT1789-100ML 100 ml
EUR 50

Rhodamine B solution, 0.2% in isopropanol
GT1789-500ML 500 ml
EUR 86

HCV NS4 recombinant antigen NS4a+b, Rhodamine
00116-V-R-01mg 0,1 mg
EUR 267.5
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Rhodamine conjugate.

HCV NS4 recombinant antigen NS4a+b, Rhodamine
00116-V-R-1000ug 1000 ug
EUR 1282.5
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Rhodamine conjugate.

HCV type a+b Nonstructural Protein 4 [Rhodamine]
DAG1423 100 µg
EUR 810

Rhodamine 800
HY-101876 50mg
EUR 173

Rhodamine 6G
HY-D0309 10mM/1mL
EUR 126

Rhodamine 123
HY-D0816 25mg
EUR 173

Rhodamine 110
HY-D0817 100mg
EUR 147

Rhodamine Phalloidin
HY-K0903 300T
EUR 843

Rhodamine 123
abx082227-5mg 5 mg
EUR 217

Ubiquitin-Rhodamine
6411-50
EUR 544

Rhodamine antibody
10C-CR2050M1 500 ug
EUR 446
Description: Mouse monoclonal Rhodamine antibody

Fmoc-b,b-diphenyl-Ala-OH
B-2725.0250 250.0mg
EUR 139
Description: Sum Formula: C30H25NO4; CAS# [201484-50-6]

Fmoc-b,b-diphenyl-Ala-OH
B-2725.1000 1.0g
EUR 357
Description: Sum Formula: C30H25NO4; CAS# [201484-50-6]

Fmoc-b,b-diphenyl-Ala-OH
B-2725.5000 5.0g
EUR 1336
Description: Sum Formula: C30H25NO4; CAS# [201484-50-6]

Recombinant Hepatitis C Virus NS4 a+b, Rhodamine Labeled
7-07327 100µg Ask for price

Recombinant Hepatitis C Virus NS4 a+b, Rhodamine Labeled
7-07328 500µg Ask for price

Recombinant Hepatitis C Virus NS4 a+b, Rhodamine Labeled
7-07329 1000µg Ask for price

Hepatitis C Virus NS4 a+b, Rhodamine Labeled Protein
20-abx260348
  • EUR 1247.00
  • EUR 467.00
  • EUR 1970.00
  • 0.5 mg
  • 100 ug
  • 1 mg

HCV Nucleocapsid [Rhodamine]
DAG1390 100 µg
EUR 810

Rhodamine phalloidin 300u
00027 300
EUR 345
Description: Minimum order quantity: 1 unit of 300

Rhodamine 110 phalloidin
00032 300ST
EUR 345
Description: Minimum order quantity: 1 unit of 300ST

Rhodamine-DHPE: (5mg)
60026 5MG
EUR 226
Description: Minimum order quantity: 1 unit of 5MG

Rhodamine 123 (chloride)
C3140-10 10 mg
EUR 112
Description: Rhodamine 123 is a fluorescent tracer dye used as substrate for P-glycoprotein.Fluorescent tracer dyes represent a class of sub-cellular probes allowing the examination of cellular processes in real-time with minimal impact upon such processes.

Rhodamine 123 (chloride)
C3140-100 100 mg
EUR 398
Description: Rhodamine 123 is a fluorescent tracer dye used as substrate for P-glycoprotein.Fluorescent tracer dyes represent a class of sub-cellular probes allowing the examination of cellular processes in real-time with minimal impact upon such processes.

Rhodamine 123 (chloride)
C3140-25 25 mg
EUR 163
Description: Rhodamine 123 is a fluorescent tracer dye used as substrate for P-glycoprotein.Fluorescent tracer dyes represent a class of sub-cellular probes allowing the examination of cellular processes in real-time with minimal impact upon such processes.

Recombinant hSUMO2-Rhodamine
6414-50
EUR 544

Biotin antibody (rhodamine)
60R-1004 1 mg
EUR 206
Description: Biotin antibody (rhodamine)

Streptavidin protein (Rhodamine)
65C-CE0311 1 mg
EUR 275
Description: Purified homogeneous preparation of rhodamine conjugated Streptavidin protein

Fmoc-b-Ala-OH
B-1025.0005 5.0g
EUR 115
Description: Sum Formula: C18H17NO4; CAS# [35737-10-1]

Fmoc-b-Ala-OH
B-1025.0025 25.0g
EUR 236
Description: Sum Formula: C18H17NO4; CAS# [35737-10-1]

Fmoc-b-Ala-OH
B-1025.0100 100.0g
EUR 635
Description: Sum Formula: C18H17NO4; CAS# [35737-10-1]

Fmoc-b-Ala-OPfp
B-1765.0005 5.0g
EUR 236
Description: Sum Formula: C24H16F5NO4; CAS# [149303-38-8]

Fmoc-b-Ala-OPfp
B-1765.0025 25.0g
EUR 841
Description: Sum Formula: C24H16F5NO4; CAS# [149303-38-8]

Absolute Mag Rhodamine B Fluorescent Iron Oxide Nanoparticles, 10 nm
WNM-X061 2 mL
EUR 689

Fmoc-b-cyclohexyl-Ala-OH
B-1975.0001 1.0g
EUR 126
Description: Sum Formula: C24H27NO4; CAS# [135673-97-1]

Fmoc-b-cyclohexyl-Ala-OH
B-1975.0005 5.0g
EUR 418
Description: Sum Formula: C24H27NO4; CAS# [135673-97-1]

Fmoc-b-cyclohexyl-Ala-OH
B-1975.0025 25.0g
EUR 1192
Description: Sum Formula: C24H27NO4; CAS# [135673-97-1]

Fmoc-b-cyclopropyl-Ala-OH
B-2905.0001 1.0g
EUR 345
Description: Sum Formula: C21H21NO4; CAS# [214750-76-2]

Fmoc-b-cyclopropyl-Ala-OH
B-2905.0005 5.0g
EUR 1301
Description: Sum Formula: C21H21NO4; CAS# [214750-76-2]

Fmoc-b-(2-thienyl)-Ala-OH
B-1665.0001 1.0g
EUR 284
Description: Sum Formula: C22H19NO4S; CAS# [130309-35-2]

Fmoc-b-(2-thienyl)-Ala-OH
B-1665.0005 5.0g
EUR 1046
Description: Sum Formula: C22H19NO4S; CAS# [130309-35-2]

Fmoc-b-(3-pyridyl)-Ala-OH
B-2005.0001 1.0g
EUR 393
Description: Sum Formula: C23H20N2O4; CAS# [175453-07-3]

Fmoc-b-(3-pyridyl)-Ala-OH
B-2005.0005 5.0g
EUR 1482
Description: Sum Formula: C23H20N2O4; CAS# [175453-07-3]

Fmoc-b-cyclohexyl-D-Ala-OH
B-2345.0001 1.0g
EUR 151
Description: Sum Formula: C24H27NO4; CAS# [144701-25-7]

Fmoc-b-cyclohexyl-D-Ala-OH
B-2345.0005 5.0g
EUR 515
Description: Sum Formula: C24H27NO4; CAS# [144701-25-7]

Fmoc-b-cyclohexyl-D-Ala-OH
B-2345.0025 25.0g
EUR 1965
Description: Sum Formula: C24H27NO4; CAS# [144701-25-7]

Fmoc-b-(3-benzothienyl)-Ala-OH
B-2830.0001 1.0g
EUR 212
Description: Sum Formula: C26H21NO4S; CAS# [177966-60-8]

Fmoc-b-(3-benzothienyl)-Ala-OH
B-2830.0005 5.0g
EUR 756
Description: Sum Formula: C26H21NO4S; CAS# [177966-60-8]

Fmoc-b-cyclopropyl-D-Ala-OH
B-2915.0001 1.0g
EUR 587
Description: Sum Formula: C21H21NO4; CAS# [170642-29-2]

Fmoc-b-cyclopropyl-D-Ala-OH
B-2915.0005 5.0g
EUR 2255
Description: Sum Formula: C21H21NO4; CAS# [170642-29-2]

Fmoc-b-(2-quinolyl)-Ala-OH
B-3165.0250 250.0mg
EUR 381
Description: Sum Formula: C27H22N2O4; CAS# [214852-56-9]

Fmoc-b-(2-quinolyl)-Ala-OH
B-3165.1000 1.0g
EUR 1070
Description: Sum Formula: C27H22N2O4; CAS# [214852-56-9]

Fmoc-b-azido-Aib-OH · BHA
B-4530.0001 1.0g
EUR 705
Description: Sum Formula: C19H18N4O4·C13H13N; CAS# [1926163-90-7] net

Absolute Mag Amine Dextran Magnetic Particles, Fluorescent Rhodamine B, 35 nm
WHM-P005 0.5 mL, 1 mL Ask for price

Absolute Mag Carboxyl Dextran Magnetic Particles, Fluorescent Rhodamine B, 35 nm
WHM-P013 2 mL
EUR 1188

Recombinant (E.Coli) Hepatitis C Virus (HCV) NS4 a+b, Rhodamine Labeled
RP-484 50 ug
EUR 286

Streptavidin-Rhodamine (TRITC) conjugate
20368 0.5 mg
EUR 202

Rhodamine 110 (high purity)
80103 25MG
EUR 88
Description: Minimum order quantity: 1 unit of 25MG

LC3A Rhodamine 110, CF
PR15106CF 50 ug
EUR 753

Rhodamine 6G (C.I. 45160)
GT5023-100G 100 g
EUR 90

Rhodamine 6G (C.I. 45160)
GT5023-25G 25 g
EUR 49

Rhodamine 6G (C.I. 45160)
GT5023-5G 5 g
EUR 41

Protein A antibody (rhodamine)
60C-CF0602 1 mg
EUR 117
Description: Goat polyclonal Protein A antibody (rhodamine) conjugated

Fmoc-b-(3-pyridyl)-D-Ala-OH
B-2040.0001 1.0g
EUR 273
Description: Sum Formula: C23H20N2O4; CAS# [142994-45-4]

Fmoc-b-(3-pyridyl)-D-Ala-OH
B-2040.0005 5.0g
EUR 998
Description: Sum Formula: C23H20N2O4; CAS# [142994-45-4]

Fmoc-b-(2-thienyl)-D-Ala-OH
B-2120.0001 1.0g
EUR 309
Description: Sum Formula: C22H19NO4S; CAS# [201532-42-5]

Fmoc-b-(2-thienyl)-D-Ala-OH
B-2120.0005 5.0g
EUR 1143
Description: Sum Formula: C22H19NO4S; CAS# [201532-42-5]

Fmoc-b-(2-quinolyl)-D-Ala-OH
B-3170.0250 250.0mg
EUR 441
Description: Sum Formula: C27H22N2O4; CAS# [214852-58-1]

Fmoc-b-(2-quinolyl)-D-Ala-OH
B-3170.1000 1.0g
EUR 1276
Description: Sum Formula: C27H22N2O4; CAS# [214852-58-1]

Fmoc-b-(3-benzothienyl)-D-Ala-OH
B-3435.0001 1.0g
EUR 334
Description: Sum Formula: C26H21NO4S; CAS# [177966-61-9]

Fmoc-b-(3-benzothienyl)-D-Ala-OH
B-3435.0005 5.0g
EUR 1240
Description: Sum Formula: C26H21NO4S; CAS# [177966-61-9]

Fmoc-b-(1-piperazinyl)-Ala(Boc)-OH
B-3725.0250 250.0mg
EUR 383
Description: Sum Formula: C27H33N3O6; CAS# [313052-20-9]

Fmoc-b-(1-piperazinyl)-Ala(Boc)-OH
B-3725.1000 1.0g
EUR 1088
Description: Sum Formula: C27H33N3O6; CAS# [313052-20-9]

Fmoc-b-azido-D-Aib-OH · BHA
B-4535.0001 1.0g
EUR 705
Description: Sum Formula: C19H18N4O4·C13H13N; CAS# [1926163-91-8] net

EZClick? Palmitoylated Protein Assay Kit (FACS/Microscopy), Red Fluorescence
K416-100
EUR 490

EZClick? Stearoylated Protein Assay Kit (FACS/Microscopy), Red Fluorescence
K418-100
EUR 490

The imply age of the members was 36.41 ± 6.379. Male getting old with out impression of confounders was correlated with a lower in sperm focus and motility and an elevated in sperm necrosis. Sperm focus and progressive motility had been negatively associated to the presence of confounders as wine consumption (sperm motility), urogenital an infection (sperm focus and motility), varicocele (sperm focus) and consanguinity (sperm motility). Urogenital an infection, varicocele and consanguinity positively correlated with sperm necrosis. The most necessary discovering was the statement of a unfavourable impact of male getting old on sperm parameters resembling focus, motility, and viability.

It is feasible to hypothesize age-dependent adjustments of testicular setting, in all probability associated to reactive oxygen species manufacturing. The demonstration, in a big pattern of sufferers, that getting old influences sperm high quality strongly motivates additional analysis centered on the mechanisms concerned in this phenomenon and its results on offspring health. We talk about challenges and alternatives to combine this method with multibeam scanning electron microscopy workflows in addition to a number of imaging modalities for mapping of organ and tissue construction and perform. Semen evaluation was carried out by mild microscopy and transmission electron microscopy mathematically elaborated.