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Electron Microscopy Sample Preparation Protocol Enabling Nano-to-mesoscopic Mapping of Cellular Connectomes and Their Habitats in Human Tissues and Organs

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Electron Microscopy Sample Preparation Protocol Enabling Nano-to-mesoscopic Mapping of Cellular Connectomes and Their Habitats in Human Tissues and Organs

Multibeam scanning electron microscopy (multiSEM) gives a technical platform for seamless nano-to-mesoscale mapping of cells in human tissues and organs, which is a serious new initiative of the U.S. National Institutes of Health. Such cross-length-scale imaging is anticipated to supply unprecedented understanding of relationships between mobile well being and tissue-organ in addition to organismal-scale well being outcomes. For instance, understanding relationships between loss in cell viability and cell community connectivity allows identification of emergent behaviors and prediction of degenerative illness onset, in organs as numerous as bone and mind, at early timepoints, offering a foundation for future therapies and prevention.

Developed for fast throughput imaging of minute defects on semiconductor wafers, multiSEM has just lately been tailored for imaging of human organs, their constituent tissues, and their respective mobile inhabitants. Through integration of geospatial approaches, statistical and community modelling, advances in computing and the administration of immense datasets, in addition to current developments in machine studying that allow the automation of large knowledge analyses, multiSEM and different cross- reducing imaging applied sciences have the potential to exert a profound impression on elucidation of illness mechanisms, translating to enhancements in human well being. Here we offer a protocol for acquisition and preparation of pattern specimen sizes of diagnostic relevance for human anatomy and physiology.

Activation of sort 1 cannabinoid (CB1) receptors by endogenous, exogenous (hashish derivatives) or artificial cannabinoids (i.e., CP 55.940, Win-2) has all kinds of behavioral results because of the presence of CB1 receptors in the mind. In situ hybridization and immunohistochemical methods have been essential for outlining the CB1 receptor expression and localization on the mobile stage. Nevertheless, extra superior strategies are wanted to disclose the exact topography of CB1 receptors in the mind, particularly in unsuspected websites resembling different cell varieties and organelles with low receptor expression (e.g., glutamatergic neurons, astrocytes, mitochondria).

High-resolution immunoelectron microscopy gives a extra exact detection technique for the subcellular localization of CB1 receptors in the mind. Herein, we describe a single pre-embedding immunogold technique for electron microscopy primarily based on the use of particular CB1 receptor antibodies and silver-intensified 1.four nm gold-labeled Fab’ fragments, and a mixed pre-embedding immunogold and immunoperoxidase technique that employs biotinylated secondary antibodies and avidin-biotin-peroxidase complicated for the simultaneous localization of CB1 receptors and protein markers of particular mind cells or synapses (e.g., GFAP, GLAST, IBA-1, PSD-95, gephyrin).

Cryo-transmission Electron Microscopy of Outer-inner Membrane Vesicles Naturally Secreted by Gram-negative Pathogenic Bacteria

A protocol was developed to visualise and analyze the construction of membrane vesicles (MVs) from Gram-negative micro organism. It is now accepted that these micrometric spherical vesicles are generally produced by cells from all three domains of life, so the protocol may very well be helpful in the examine of vesicles produced by eukaryotes and archaea in addition to micro organism. The multiplicity of capabilities carried out by MVs, associated to cell communication, interplay with the immune system, pathogenesis, and nutrient acquisition, amongst others, has made MVs a sizzling matter of analysis. Due to their small measurement (25-300 nm), the statement of MVs requires electron microscopy and is often carried out by transmission electron microscopy (TEM) of negatively stained MVs.

Other protocols utilized for his or her visualization embrace scanning electron microscopy, TEM after fixation and embedding of vesicles, and even atomic power microscopy. In some of these methods, vesicle construction is altered by drying, whereas others are time-consuming and most of them can generate artifacts. Cryo-TEM after plunge freezing permits the visualization of samples embedded in a skinny movie of vitreous ice, which preserves their native mobile constructions and gives the very best accessible decision for the imaging. This is achieved by very excessive cooling charges that flip the intrinsic water of cells into vitreous ice, avoiding crystal formation and part segregation between water and solutes. In addition to different varieties of characterization, an correct information of MV construction, which could be obtained by this protocol, is important for MV utility in totally different fields.

Electron Microscopy Sample Preparation Protocol Enabling Nano-to-mesoscopic Mapping of Cellular Connectomes and Their Habitats in Human Tissues and Organs

Influence of age on sperm traits evaluated by mild and electron microscopies

The impression of male getting old on male fertility has solely just lately turn out to be of curiosity to the scientific group. This examine goals to evaluate the connection between age and fertility amongst a pattern of males, contemplating the person and pathological traits. In this retrospective examine knowledge of semen evaluation and medical historical past of 1294 Italian male sufferers had been thought-about.  A generalized linear mannequin was used to discover the affect of male age on semen high quality, contemplating as confounders wine consumption, smoking habits, presence of varicocele, consanguinity and optimistic semen bacteriological evaluation and urethral swab.

Rhodamine B
HY-Y0016 1g
EUR 160

Rhodamine B
TBP03900 6X100mg
EUR 257

Rhodamine B
RB0464 100g
EUR 69.14
  • Product category: Biochemicals/Indicators/Stains/Biological Stains

Octadecyl rhodamine B
60033 10MG
EUR 281
Description: Minimum order quantity: 1 unit of 10MG

Rhodamine B [HRP]
DAGA-060H 1mg
EUR 1209

Rhodamine B [BSA]
DAGA-072B 1mg
EUR 780

Rhodamine B [KLH]
DAGA-078K 1mg
EUR 1079

Rhodamine B base
GT5360-100G 100 g
EUR 158

Rhodamine B base
GT5360-10G 10 g
EUR 50

Rhodamine B base
GT5360-25G 25 g
EUR 70

Rhodamine B base
GT5360-50G 50 g
EUR 102

Rhodamine B ELISA Kit
DEIANJ26 96T
EUR 866
Description: The Rhodamine B ELISA Kit is an immunoassay for the quantitative and sensitive screening of Rhodamine B in food samples such as chili sauce and chili powder, etc.

Rhodamine B (RDB) Antibody
20-abx179008
  • EUR 439.00
  • EUR 133.00
  • EUR 1274.00
  • EUR 606.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Rhodamine B (C.I. 45170)
GT7006-100G 100 g
EUR 62

Rhodamine B (C.I. 45170)
GT7006-250G 250 g
EUR 94

Rhodamine B (C.I. 45170)
GT7006-25G 25 g
EUR 46

Fluorescence Microscopy Aid
4400 3 ml
EUR 180.5
Description: This is Counter - Stain/Blocking Diluent with optimal formula for preparaiton of final dilutions of FITC conjugate prior to use in FA procedures.

Rhodamine B, hexyl ester, perchlorate
22211 10 mg
EUR 115
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

OVA Conjugated Rhodamine B (RDB)
4-CPO062Ge21
  • EUR 413.60
  • EUR 214.00
  • EUR 1276.00
  • EUR 492.00
  • EUR 884.00
  • EUR 340.00
  • EUR 3040.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Inquire
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 479.0g/mol
  • Isoelectric Point: Inquire
Description: Recombinant Pan-species Rhodamine B expressed in: E.coli

Rhodamine B (RDB) Protein (OVA)
20-abx651895
  • EUR 578.00
  • EUR 258.00
  • EUR 1720.00
  • EUR 690.00
  • EUR 425.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Rhodamine B solution, 0.2% in isopropanol
GT1789-100ML 100 ml
EUR 50

Rhodamine B solution, 0.2% in isopropanol
GT1789-500ML 500 ml
EUR 86

HCV NS4 recombinant antigen NS4a+b, Rhodamine
00116-V-R-01mg 0,1 mg
EUR 267.5
  • Category: Antigens, HCV, Ag
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Rhodamine conjugate.

HCV NS4 recombinant antigen NS4a+b, Rhodamine
00116-V-R-1000ug 1000 ug
EUR 1282.5
  • Category: Antigens, HCV, Ag
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Rhodamine conjugate.

HCV type a+b Nonstructural Protein 4 [Rhodamine]
DAG1423 100 µg
EUR 810

Rhodamine antibody
10C-CR2050M1 500 ug
EUR 446
Description: Mouse monoclonal Rhodamine antibody

Ubiquitin-Rhodamine
6411-50
EUR 544

Rhodamine 123
abx082227-5mg 5 mg
EUR 217
  • Shipped within 5-10 working days.

Rhodamine 6G
HY-D0309 10mM/1mL
EUR 126

Rhodamine 123
HY-D0816 25mg
EUR 173

Rhodamine 110
HY-D0817 100mg
EUR 147

Rhodamine Phalloidin
HY-K0903 300T
EUR 843

Rhodamine 800
HY-101876 50mg
EUR 173

Fmoc-b,b-diphenyl-Ala-OH
B-2725.0250 250.0mg
EUR 139
Description: Sum Formula: C30H25NO4; CAS# [201484-50-6]

Fmoc-b,b-diphenyl-Ala-OH
B-2725.1000 1.0g
EUR 357
Description: Sum Formula: C30H25NO4; CAS# [201484-50-6]

Fmoc-b,b-diphenyl-Ala-OH
B-2725.5000 5.0g
EUR 1336
Description: Sum Formula: C30H25NO4; CAS# [201484-50-6]

Recombinant Hepatitis C Virus NS4 a+b, Rhodamine Labeled
7-07327 100µg Ask for price

Recombinant Hepatitis C Virus NS4 a+b, Rhodamine Labeled
7-07328 500µg Ask for price

Recombinant Hepatitis C Virus NS4 a+b, Rhodamine Labeled
7-07329 1000µg Ask for price

Hepatitis C Virus NS4 a+b, Rhodamine Labeled Protein
20-abx260348
  • EUR 1247.00
  • EUR 467.00
  • EUR 1970.00
  • 0.5 mg
  • 100 ug
  • 1 mg
  • Shipped within 5-10 working days.

Streptavidin protein (Rhodamine)
65C-CE0311 1 mg
EUR 275
Description: Purified homogeneous preparation of rhodamine conjugated Streptavidin protein

Rhodamine phalloidin 300u
00027 300
EUR 345
Description: Minimum order quantity: 1 unit of 300

Rhodamine 110 phalloidin
00032 300ST
EUR 345
Description: Minimum order quantity: 1 unit of 300ST

Rhodamine-DHPE: (5mg)
60026 5MG
EUR 226
Description: Minimum order quantity: 1 unit of 5MG

Biotin antibody (rhodamine)
60R-1004 1 mg
EUR 206
Description: Biotin antibody (rhodamine)

Recombinant hSUMO2-Rhodamine
6414-50
EUR 544

HCV Nucleocapsid [Rhodamine]
DAG1390 100 µg
EUR 810

Rhodamine 123 (chloride)
C3140-10 10 mg
EUR 112
Description: Rhodamine 123 is a fluorescent tracer dye used as substrate for P-glycoprotein.Fluorescent tracer dyes represent a class of sub-cellular probes allowing the examination of cellular processes in real-time with minimal impact upon such processes.

Rhodamine 123 (chloride)
C3140-100 100 mg
EUR 398
Description: Rhodamine 123 is a fluorescent tracer dye used as substrate for P-glycoprotein.Fluorescent tracer dyes represent a class of sub-cellular probes allowing the examination of cellular processes in real-time with minimal impact upon such processes.

Rhodamine 123 (chloride)
C3140-25 25 mg
EUR 163
Description: Rhodamine 123 is a fluorescent tracer dye used as substrate for P-glycoprotein.Fluorescent tracer dyes represent a class of sub-cellular probes allowing the examination of cellular processes in real-time with minimal impact upon such processes.

Fmoc-b-Ala-OH
B-1025.0005 5.0g
EUR 115
Description: Sum Formula: C18H17NO4; CAS# [35737-10-1]

Fmoc-b-Ala-OH
B-1025.0025 25.0g
EUR 236
Description: Sum Formula: C18H17NO4; CAS# [35737-10-1]

Fmoc-b-Ala-OH
B-1025.0100 100.0g
EUR 635
Description: Sum Formula: C18H17NO4; CAS# [35737-10-1]

Fmoc-b-Ala-OPfp
B-1765.0005 5.0g
EUR 236
Description: Sum Formula: C24H16F5NO4; CAS# [149303-38-8]

Fmoc-b-Ala-OPfp
B-1765.0025 25.0g
EUR 841
Description: Sum Formula: C24H16F5NO4; CAS# [149303-38-8]

Absolute Mag Rhodamine B Fluorescent Iron Oxide Nanoparticles, 10 nm
WNM-X061 2 mL
EUR 689

Fmoc-b-cyclopropyl-Ala-OH
B-2905.0001 1.0g
EUR 345
Description: Sum Formula: C21H21NO4; CAS# [214750-76-2]

Fmoc-b-cyclopropyl-Ala-OH
B-2905.0005 5.0g
EUR 1301
Description: Sum Formula: C21H21NO4; CAS# [214750-76-2]

Fmoc-b-cyclohexyl-Ala-OH
B-1975.0001 1.0g
EUR 126
Description: Sum Formula: C24H27NO4; CAS# [135673-97-1]

Fmoc-b-cyclohexyl-Ala-OH
B-1975.0005 5.0g
EUR 418
Description: Sum Formula: C24H27NO4; CAS# [135673-97-1]

Fmoc-b-cyclohexyl-Ala-OH
B-1975.0025 25.0g
EUR 1192
Description: Sum Formula: C24H27NO4; CAS# [135673-97-1]

Recombinant (E.Coli) Hepatitis C Virus (HCV) NS4 a+b, Rhodamine Labeled
RP-484 50 ug
EUR 286

Absolute Mag Amine Dextran Magnetic Particles, Fluorescent Rhodamine B, 35 nm
WHM-P005 0.5 mL, 1 mL Ask for price

Absolute Mag Carboxyl Dextran Magnetic Particles, Fluorescent Rhodamine B, 35 nm
WHM-P013 2 mL
EUR 1188

Fmoc-b-(3-benzothienyl)-Ala-OH
B-2830.0001 1.0g
EUR 212
Description: Sum Formula: C26H21NO4S; CAS# [177966-60-8]

Fmoc-b-(3-benzothienyl)-Ala-OH
B-2830.0005 5.0g
EUR 756
Description: Sum Formula: C26H21NO4S; CAS# [177966-60-8]

Fmoc-b-cyclopropyl-D-Ala-OH
B-2915.0001 1.0g
EUR 587
Description: Sum Formula: C21H21NO4; CAS# [170642-29-2]

Fmoc-b-cyclopropyl-D-Ala-OH
B-2915.0005 5.0g
EUR 2255
Description: Sum Formula: C21H21NO4; CAS# [170642-29-2]

Fmoc-b-(2-quinolyl)-Ala-OH
B-3165.0250 250.0mg
EUR 381
Description: Sum Formula: C27H22N2O4; CAS# [214852-56-9]

Fmoc-b-(2-quinolyl)-Ala-OH
B-3165.1000 1.0g
EUR 1070
Description: Sum Formula: C27H22N2O4; CAS# [214852-56-9]

Fmoc-b-azido-Aib-OH · BHA
B-4530.0001 1.0g
EUR 705
Description: Sum Formula: C19H18N4O4·C13H13N; CAS# [1926163-90-7] net

Fmoc-b-(2-thienyl)-Ala-OH
B-1665.0001 1.0g
EUR 284
Description: Sum Formula: C22H19NO4S; CAS# [130309-35-2]

Fmoc-b-(2-thienyl)-Ala-OH
B-1665.0005 5.0g
EUR 1046
Description: Sum Formula: C22H19NO4S; CAS# [130309-35-2]

Fmoc-b-(3-pyridyl)-Ala-OH
B-2005.0001 1.0g
EUR 393
Description: Sum Formula: C23H20N2O4; CAS# [175453-07-3]

Fmoc-b-(3-pyridyl)-Ala-OH
B-2005.0005 5.0g
EUR 1482
Description: Sum Formula: C23H20N2O4; CAS# [175453-07-3]

Fmoc-b-cyclohexyl-D-Ala-OH
B-2345.0001 1.0g
EUR 151
Description: Sum Formula: C24H27NO4; CAS# [144701-25-7]

Fmoc-b-cyclohexyl-D-Ala-OH
B-2345.0005 5.0g
EUR 515
Description: Sum Formula: C24H27NO4; CAS# [144701-25-7]

Fmoc-b-cyclohexyl-D-Ala-OH
B-2345.0025 25.0g
EUR 1965
Description: Sum Formula: C24H27NO4; CAS# [144701-25-7]

Streptavidin-Rhodamine (TRITC) conjugate
20368 0.5 mg
EUR 202

Protein A antibody (rhodamine)
60C-CF0602 1 mg
EUR 117
Description: Goat polyclonal Protein A antibody (rhodamine) conjugated

Rhodamine 110 (high purity)
80103 25MG
EUR 88
Description: Minimum order quantity: 1 unit of 25MG

Rhodamine 6G (C.I. 45160)
GT5023-100G 100 g
EUR 90

Rhodamine 6G (C.I. 45160)
GT5023-25G 25 g
EUR 49

Rhodamine 6G (C.I. 45160)
GT5023-5G 5 g
EUR 41

LC3A Rhodamine 110, CF
PR15106CF 50 ug
EUR 753

Fmoc-b-(2-quinolyl)-D-Ala-OH
B-3170.0250 250.0mg
EUR 441
Description: Sum Formula: C27H22N2O4; CAS# [214852-58-1]

Fmoc-b-(2-quinolyl)-D-Ala-OH
B-3170.1000 1.0g
EUR 1276
Description: Sum Formula: C27H22N2O4; CAS# [214852-58-1]

Fmoc-b-(3-benzothienyl)-D-Ala-OH
B-3435.0001 1.0g
EUR 334
Description: Sum Formula: C26H21NO4S; CAS# [177966-61-9]

Fmoc-b-(3-benzothienyl)-D-Ala-OH
B-3435.0005 5.0g
EUR 1240
Description: Sum Formula: C26H21NO4S; CAS# [177966-61-9]

Fmoc-b-(1-piperazinyl)-Ala(Boc)-OH
B-3725.0250 250.0mg
EUR 383
Description: Sum Formula: C27H33N3O6; CAS# [313052-20-9]

Fmoc-b-(1-piperazinyl)-Ala(Boc)-OH
B-3725.1000 1.0g
EUR 1088
Description: Sum Formula: C27H33N3O6; CAS# [313052-20-9]

Fmoc-b-azido-D-Aib-OH · BHA
B-4535.0001 1.0g
EUR 705
Description: Sum Formula: C19H18N4O4·C13H13N; CAS# [1926163-91-8] net

Fmoc-b-(3-pyridyl)-D-Ala-OH
B-2040.0001 1.0g
EUR 273
Description: Sum Formula: C23H20N2O4; CAS# [142994-45-4]

Fmoc-b-(3-pyridyl)-D-Ala-OH
B-2040.0005 5.0g
EUR 998
Description: Sum Formula: C23H20N2O4; CAS# [142994-45-4]

Fmoc-b-(2-thienyl)-D-Ala-OH
B-2120.0001 1.0g
EUR 309
Description: Sum Formula: C22H19NO4S; CAS# [201532-42-5]

Fmoc-b-(2-thienyl)-D-Ala-OH
B-2120.0005 5.0g
EUR 1143
Description: Sum Formula: C22H19NO4S; CAS# [201532-42-5]

EZClick? Myristoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence
K497-100
EUR 490

EZClick? Palmitoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence
K452-100
EUR 490

EZClick? Stearoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence
K453-100
EUR 490

EZClick? Palmitoylated Protein Assay Kit (FACS/Microscopy), Red Fluorescence
K416-100
EUR 490

EZClick? Stearoylated Protein Assay Kit (FACS/Microscopy), Red Fluorescence
K418-100
EUR 490

Fmoc-Trp(For)-OH
B-3710.0001 1.0g
EUR 115
Description: Sum Formula: C27H22N2O5; CAS# [152338-45-9]

Fmoc-Trp(For)-OH
B-3710.0005 5.0g
EUR 368
Description: Sum Formula: C27H22N2O5; CAS# [152338-45-9]

Fmoc-Lys(For)-OH
B-1865.0001 1.0g
EUR 115
Description: Sum Formula: C22H24N2O5; CAS# [201004-23-1]

Fmoc-Lys(For)-OH
B-1865.0005 5.0g
EUR 368
Description: Sum Formula: C22H24N2O5; CAS# [201004-23-1]

Fmoc-Lys(For)-OH
B-1865.0025 25.0g
EUR 1360
Description: Sum Formula: C22H24N2O5; CAS# [201004-23-1]

Recombinant Hepatitis C HCV NS4 a+b Rhodamine Protein, Untagged, E.coli-100ug
QP12179-100ug 100ug
EUR 318

Recombinant Hepatitis C HCV NS4 a+b Rhodamine Protein, Untagged, E.coli-1mg
QP12179-1mg 1mg
EUR 1561

Recombinant Hepatitis C HCV NS4 a+b Rhodamine Protein, Untagged, E.coli-500ug
QP12179-500ug 500ug
EUR 962

Absolute Mag Carboxyl & Amine Dextran Magnetic Particles, Fluorescent Rhodamine B, 50 nm
WHM-P015 2 mL
EUR 1188

Holder for Plasmid Midi, Maxi and Maxi plus, Ion Exchange column
FAPDE-holder-for-ion-exchange 1 prep
EUR 158

Fmoc-Asn(GlcNAc(Ac)3-b-D)-OH
B-2480.0100 100.0mg
EUR 587
Description: Sum Formula: C33H37N3O13; CAS# [131287-39-3]

Fmoc-Asn(GlcNAc(Ac)3-b-D)-OH
B-2480.0250 250.0mg
EUR 1143
Description: Sum Formula: C33H37N3O13; CAS# [131287-39-3]

Fmoc-N-(N-b-Boc-aminoethyl)-Gly-OH
B-3285.0001 1.0g
EUR 454
Description: Sum Formula: C24H28N2O6; CAS# [141743-15-9]

Fmoc-N-(N-b-Boc-aminoethyl)-Gly-OH
B-3285.0005 5.0g
EUR 1698
Description: Sum Formula: C24H28N2O6; CAS# [141743-15-9]

Fmoc-Ser(GlcNAc(Ac)3-b-D)-OH
B-3865.0025 25.0mg
EUR 265
Description: Sum Formula: C32H36N2O13; CAS# [160067-63-0]

Fmoc-Ser(GlcNAc(Ac)3-b-D)-OH
B-3865.0100 100.0mg
EUR 735
Description: Sum Formula: C32H36N2O13; CAS# [160067-63-0]

Fmoc-Ser(GlcNAc(Ac)3-b-D)-OH
B-3865.0250 250.0mg
EUR 1438
Description: Sum Formula: C32H36N2O13; CAS# [160067-63-0]

Fmoc-Ser(GalNAc(Ac)3-b-D)-OH
B-4235.0025 25.0mg
EUR 265
Description: Sum Formula: C32H36N2O13; CAS# [1676104-71-4]

Fmoc-Ser(GalNAc(Ac)3-b-D)-OH
B-4235.0100 100.0mg
EUR 735
Description: Sum Formula: C32H36N2O13; CAS# [1676104-71-4]

Fmoc-Ser(GalNAc(Ac)3-b-D)-OH
B-4235.0250 250.0mg
EUR 1438
Description: Sum Formula: C32H36N2O13; CAS# [1676104-71-4]

Fmoc-Thr(GalNAc(Ac)3-b-D)-OH
B-4240.0025 25.0mg
EUR 277
Description: Sum Formula: C33H38N2O13; CAS# [133575-43-6]

Fmoc-Thr(GalNAc(Ac)3-b-D)-OH
B-4240.0100 100.0mg
EUR 770
Description: Sum Formula: C33H38N2O13; CAS# [133575-43-6]

Fmoc-Thr(GalNAc(Ac)3-b-D)-OH
B-4240.0250 250.0mg
EUR 1510
Description: Sum Formula: C33H38N2O13; CAS# [133575-43-6]

Fmoc-Thr(GlcNAc(Ac)3-b-D)-OH
B-4245.0025 25.0mg
EUR 277
Description: Sum Formula: C33H38N2O13; CAS# [160168-40-1]

Fmoc-Thr(GlcNAc(Ac)3-b-D)-OH
B-4245.0100 100.0mg
EUR 770
Description: Sum Formula: C33H38N2O13; CAS# [160168-40-1]

Fmoc-Thr(GlcNAc(Ac)3-b-D)-OH
B-4245.0250 250.0mg
EUR 1510
Description: Sum Formula: C33H38N2O13; CAS# [160168-40-1]

R110-PEG (Rhodamine 110-PEG)
10225 5MG
EUR 245
Description: Minimum order quantity: 1 unit of 5MG

HCV core recombinant antigen, Rhodamine
00115-V-R-01mg 0,1 mg
EUR 267.5
  • Category: Antigens, HCV, Ag
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa . Rhodamine conjugate.

HCV core recombinant antigen, Rhodamine
00115-V-R-1000ug 1000 ug
EUR 1282.5
  • Category: Antigens, HCV, Ag
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa . Rhodamine conjugate.

Rabbit anti Bovine IgG (rhodamine)
43C-CB0117 1.5 mg
EUR 302
Description: Rabbit anti Bovine IgG secondary antibody (Rhodamine)

Rabbit anti Bovine IgG (rhodamine)
43C-CB0131 2 mg
EUR 302
Description: Rabbit anti Bovine IgG secondary antibody (Rhodamine)

Goat anti Bovine IgG (rhodamine)
43C-CB0138 2 mg
EUR 283
Description: Goat anti Bovine IgG secondary antibody (Rhodamine)

Goat anti Cat IgG (rhodamine)
43C-CB0217 2 mg
EUR 339
Description: Goat anti Cat IgG secondary antibody (Rhodamine)

Rabbit anti Cat IgG (rhodamine)
43C-CB0224 2 mg
EUR 299
Description: Rabbit anti Cat IgG secondary antibody (Rhodamine)

Goat anti Cat IgG (rhodamine)
43C-CB0231 2 mg
EUR 301
Description: Goat anti Cat IgG secondary antibody (Rhodamine)

Rabbit anti Chicken IgG (rhodamine)
43C-CB0317 1.5 mg
EUR 302
Description: Rabbit anti Chicken IgG secondary antibody (Rhodamine)

Rabbit anti Chicken IgG (rhodamine)
43C-CB0324 1.5 mg
EUR 315
Description: Rabbit anti Chicken IgG secondary antibody (Rhodamine)

Rabbit anti Dog IgG (rhodamine)
43C-CB0410 1.5 mg
EUR 318
Description: Rabbit anti Dog IgG secondary antibody (Rhodamine)

Rabbit anti Dog IgG (rhodamine)
43C-CB0417 2 mg
EUR 302
Description: Rabbit anti Dog IgG secondary antibody (Rhodamine)

Rabbit anti Goat IgG (rhodamine)
43C-CB0524 2 mg
EUR 291
Description: Rabbit anti Goat IgG secondary antibody (Rhodamine)

Rabbit anti Goat IgG (rhodamine)
43C-CB0531 2 mg
EUR 291
Description: Rabbit anti Goat IgG secondary antibody (Rhodamine)

Rabbit anti Goat IgG (rhodamine)
43C-CB0538 1 mg
EUR 284
Description: Rabbit anti Goat IgG secondary antibody (Rhodamine)

The imply age of the members was 36.41 ± 6.379. Male getting old with out impression of confounders was correlated with a lower in sperm focus and motility and an elevated in sperm necrosis. Sperm focus and progressive motility had been negatively associated to the presence of confounders as wine consumption (sperm motility), urogenital an infection (sperm focus and motility), varicocele (sperm focus) and consanguinity (sperm motility). Urogenital an infection, varicocele and consanguinity positively correlated with sperm necrosis. The most necessary discovering was the statement of a unfavourable impact of male getting old on sperm parameters resembling focus, motility, and viability.

It is feasible to hypothesize age-dependent adjustments of testicular setting, in all probability associated to reactive oxygen species manufacturing. The demonstration, in a big pattern of sufferers, that getting old influences sperm high quality strongly motivates additional analysis centered on the mechanisms concerned in this phenomenon and its results on offspring health. We talk about challenges and alternatives to combine this method with multibeam scanning electron microscopy workflows in addition to a number of imaging modalities for mapping of organ and tissue construction and perform. Semen evaluation was carried out by mild microscopy and transmission electron microscopy mathematically elaborated.