Electron Microscopy Sample Preparation Protocol Enabling Nano-to-mesoscopic Mapping of Cellular Connectomes and Their Habitats in Human Tissues and Organs

Electron Microscopy Sample Preparation Protocol Enabling Nano-to-mesoscopic Mapping of Cellular Connectomes and Their Habitats in Human Tissues and Organs

Multibeam scanning electron microscopy (multiSEM) gives a technical platform for seamless nano-to-mesoscale mapping of cells in human tissues and organs, which is a serious new initiative of the U.S. National Institutes of Health. Such cross-length-scale imaging is anticipated to supply unprecedented understanding of relationships between mobile well being and tissue-organ in addition to organismal-scale well being outcomes. For instance, understanding relationships between loss in cell viability and cell community connectivity allows identification of emergent behaviors and prediction of degenerative illness onset, in organs as numerous as bone and mind, at early timepoints, offering a foundation for future therapies and prevention.

Developed for fast throughput imaging of minute defects on semiconductor wafers, multiSEM has just lately been tailored for imaging of human organs, their constituent tissues, and their respective mobile inhabitants. Through integration of geospatial approaches, statistical and community modelling, advances in computing and the administration of immense datasets, in addition to current developments in machine studying that allow the automation of large knowledge analyses, multiSEM and different cross- reducing imaging applied sciences have the potential to exert a profound impression on elucidation of illness mechanisms, translating to enhancements in human well being. Here we offer a protocol for acquisition and preparation of pattern specimen sizes of diagnostic relevance for human anatomy and physiology.

Activation of sort 1 cannabinoid (CB1) receptors by endogenous, exogenous (hashish derivatives) or artificial cannabinoids (i.e., CP 55.940, Win-2) has all kinds of behavioral results because of the presence of CB1 receptors in the mind. In situ hybridization and immunohistochemical methods have been essential for outlining the CB1 receptor expression and localization on the mobile stage. Nevertheless, extra superior strategies are wanted to disclose the exact topography of CB1 receptors in the mind, particularly in unsuspected websites resembling different cell varieties and organelles with low receptor expression (e.g., glutamatergic neurons, astrocytes, mitochondria).

High-resolution immunoelectron microscopy gives a extra exact detection technique for the subcellular localization of CB1 receptors in the mind. Herein, we describe a single pre-embedding immunogold technique for electron microscopy primarily based on the use of particular CB1 receptor antibodies and silver-intensified 1.four nm gold-labeled Fab’ fragments, and a mixed pre-embedding immunogold and immunoperoxidase technique that employs biotinylated secondary antibodies and avidin-biotin-peroxidase complicated for the simultaneous localization of CB1 receptors and protein markers of particular mind cells or synapses (e.g., GFAP, GLAST, IBA-1, PSD-95, gephyrin).

Cryo-transmission Electron Microscopy of Outer-inner Membrane Vesicles Naturally Secreted by Gram-negative Pathogenic Bacteria

A protocol was developed to visualise and analyze the construction of membrane vesicles (MVs) from Gram-negative micro organism. It is now accepted that these micrometric spherical vesicles are generally produced by cells from all three domains of life, so the protocol may very well be helpful in the examine of vesicles produced by eukaryotes and archaea in addition to micro organism. The multiplicity of capabilities carried out by MVs, associated to cell communication, interplay with the immune system, pathogenesis, and nutrient acquisition, amongst others, has made MVs a sizzling matter of analysis. Due to their small measurement (25-300 nm), the statement of MVs requires electron microscopy and is often carried out by transmission electron microscopy (TEM) of negatively stained MVs.

Other protocols utilized for his or her visualization embrace scanning electron microscopy, TEM after fixation and embedding of vesicles, and even atomic power microscopy. In some of these methods, vesicle construction is altered by drying, whereas others are time-consuming and most of them can generate artifacts. Cryo-TEM after plunge freezing permits the visualization of samples embedded in a skinny movie of vitreous ice, which preserves their native mobile constructions and gives the very best accessible decision for the imaging. This is achieved by very excessive cooling charges that flip the intrinsic water of cells into vitreous ice, avoiding crystal formation and part segregation between water and solutes. In addition to different varieties of characterization, an correct information of MV construction, which could be obtained by this protocol, is important for MV utility in totally different fields.

Electron Microscopy Sample Preparation Protocol Enabling Nano-to-mesoscopic Mapping of Cellular Connectomes and Their Habitats in Human Tissues and Organs

Influence of age on sperm traits evaluated by mild and electron microscopies

The impression of male getting old on male fertility has solely just lately turn out to be of curiosity to the scientific group. This examine goals to evaluate the connection between age and fertility amongst a pattern of males, contemplating the person and pathological traits. In this retrospective examine knowledge of semen evaluation and medical historical past of 1294 Italian male sufferers had been thought-about.  A generalized linear mannequin was used to discover the affect of male age on semen high quality, contemplating as confounders wine consumption, smoking habits, presence of varicocele, consanguinity and optimistic semen bacteriological evaluation and urethral swab.

Rhodamine B

abx082254-5g 5 g
EUR 210

Rhodamine B

AG031 1 mg
EUR 627.6

Rhodamine B

HY-Y0016 1g
EUR 192

Rhodamine B

571565 25.0g
EUR 205

Rhodamine B

30108-22 25G
EUR 37.45

Rhodamine B

RB0464 100g
EUR 82.97

Rhodamine B

TBP03900 6X100mg
EUR 308.4

RHODAMINE B

R7228 100G
EUR 20.3

Rhodamine B

41840000-1 25 g
EUR 26.54

Rhodamine B

41840000-2 100 g
EUR 70.01

Rhodamine B

T7975-10mg 10mg Ask for price
Description: Rhodamine B

Rhodamine B

T7975-1g 1g Ask for price
Description: Rhodamine B

Rhodamine B

T7975-1mg 1mg Ask for price
Description: Rhodamine B

Rhodamine B

T7975-50mg 50mg Ask for price
Description: Rhodamine B

Rhodamine B

T7975-5mg 5mg Ask for price
Description: Rhodamine B

Rhodamine B (C.I. 45170)

GT7006-100G 100 g
EUR 74.4

Rhodamine B (C.I. 45170)

GT7006-250G 250 g
EUR 112.8

Rhodamine B (C.I. 45170)

GT7006-25G 25 g
EUR 55.2

Rhodamine B (C.I. 45170)

GT7006-100 100
EUR 31.7

Rhodamine B (C.I. 45170)

GT7006-25 25
EUR 15.9

Rhodamine B (C.I. 45170)

GT7006-250 250
EUR 63.3

Rhodamine B [HRP]

DAGA-060H 1mg
EUR 1450.8

Rhodamine B [BSA]

DAGA-072B 1mg
EUR 936

Rhodamine B [KLH]

DAGA-078K 1mg
EUR 1294.8

Rhodamine B base

GT5360-100G 100 g
EUR 189.6

Rhodamine B base

GT5360-10G 10 g
EUR 60

Rhodamine B base

GT5360-25G 25 g
EUR 84

Rhodamine B base

GT5360-50G 50 g
EUR 122.4

Rhodamine B Base

R01195 5G
EUR 119.67

Rhodamine B base

GT5360-10 10
EUR 19.8

Rhodamine B base

GT5360-100 100
EUR 126.5

Rhodamine B base

GT5360-25 25
EUR 39.4

Rhodamine B base

GT5360-50 50
EUR 71.1

Exosome electron microscopy imaging (EM)

S109 -
EUR 600

Rhodamine B C.I. No. 45170

R01200 100G
EUR 363.7

Lissamine rhodamine B

598993 1.0g
EUR 200

Octadecyl Rhodamine B

60033-1 EA
EUR 330

Lissamine rhodamine B

T20771-10mg 10mg Ask for price
Description: Lissamine rhodamine B

Lissamine rhodamine B

T20771-1g 1g Ask for price
Description: Lissamine rhodamine B

Lissamine rhodamine B

T20771-1mg 1mg Ask for price
Description: Lissamine rhodamine B

Lissamine rhodamine B

T20771-50mg 50mg Ask for price
Description: Lissamine rhodamine B

Lissamine rhodamine B

T20771-5mg 5mg Ask for price
Description: Lissamine rhodamine B

Rhodamine B Isothiocyanate

41840004-1 100 mg
EUR 173.72

Rhodamine B Isothiocyanate

41840004-2 250 mg
EUR 364.1

Rhodamine B ELISA Kit

DEIANJ26 96T
EUR 1039.2
Description: The Rhodamine B ELISA Kit is an immunoassay for the quantitative and sensitive screening of Rhodamine B in food samples such as chili sauce and chili powder, etc.

Rhodamine B (RDB) Antibody

20-abx179008
  • EUR 526.80
  • EUR 159.60
  • EUR 1528.80
  • EUR 727.20
  • EUR 410.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

HCV NS4 a+b Rhodamine

hcv-226 100µg
EUR 275
Description: Recombinant Hepatitis C Virus NS4 a+b, Rhodamine Labeled

Rhodamine B *CAS 81-88-9*

69-100mg 100 mg
EUR 86
Description: Rhodamine B (also called Rhodamine 610, Basic Violet 10, or C.I.

Rhodamine B (RDB) Protein (OVA)

20-abx651895
  • EUR 693.60
  • EUR 309.60
  • EUR 2064.00
  • EUR 828.00
  • EUR 510.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

OVA Conjugated Rhodamine B (RDB)

4-CPO062Ge21
  • EUR 496.32
  • EUR 256.80
  • EUR 1531.20
  • EUR 590.40
  • EUR 1060.80
  • EUR 408.00
  • EUR 3648.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Pan-species Rhodamine B expressed in: E.coli

OVA Conjugated Rhodamine B (RDB)

RPU55106-100ug 100ug
EUR 431.2

OVA Conjugated Rhodamine B (RDB)

RPU55106-1mg 1mg
EUR 1911

OVA Conjugated Rhodamine B (RDB)

RPU55106-50ug 50ug
EUR 336

OVA Conjugated Rhodamine B (RDB)

CPO062Ge21 10ug
EUR 140

Rhodamine 123

abx082227-5mg 5 mg
EUR 260.4

Rhodamine 800

HY-101876 50mg
EUR 207.6

Rhodamine 123

HY-D0816 25mg
EUR 207.6

Rhodamine 110

HY-D0817 100mg
EUR 176.4

Rhodamine 123

341325 5.0mg
EUR 180

Rhodamine 123

70010-1 EA
EUR 116

Rhodamine 700

584639 200.0mg
EUR 390

Rhodamine 123

EGY044 5 mg
EUR 90

Rhodamine 110

T16742-10mg 10mg Ask for price
Description: Rhodamine 110

Rhodamine 110

T16742-1g 1g Ask for price
Description: Rhodamine 110

Rhodamine 110

T16742-1mg 1mg Ask for price
Description: Rhodamine 110

Rhodamine 110

T16742-50mg 50mg Ask for price
Description: Rhodamine 110

Rhodamine 110

T16742-5mg 5mg Ask for price
Description: Rhodamine 110

Rhodamine 123

T16743-10mg 10mg Ask for price
Description: Rhodamine 123

Rhodamine 123

T16743-1g 1g Ask for price
Description: Rhodamine 123

Rhodamine 123

T16743-1mg 1mg Ask for price
Description: Rhodamine 123

Rhodamine 123

T16743-50mg 50mg Ask for price
Description: Rhodamine 123

Rhodamine 123

T16743-5mg 5mg Ask for price
Description: Rhodamine 123

Rhodamine 700

T34319-10mg 10mg Ask for price
Description: Rhodamine 700

Rhodamine 700

T34319-1g 1g Ask for price
Description: Rhodamine 700

Rhodamine 700

T34319-1mg 1mg Ask for price
Description: Rhodamine 700

Rhodamine 700

T34319-50mg 50mg Ask for price
Description: Rhodamine 700

Rhodamine 700

T34319-5mg 5mg Ask for price
Description: Rhodamine 700

Rhodamine 800

T19054-10mg 10mg Ask for price
Description: Rhodamine 800

Rhodamine 800

T19054-1g 1g Ask for price
Description: Rhodamine 800

Rhodamine 800

T19054-1mg 1mg Ask for price
Description: Rhodamine 800

Rhodamine 800

T19054-50mg 50mg Ask for price
Description: Rhodamine 800

Rhodamine 800

T19054-5mg 5mg Ask for price
Description: Rhodamine 800

Embedding Stubs, Polyethylene, Light Microscopy - 10mm thick

23197-1 1pkg
EUR 375

Rhodamine-123

E37F22210 10mg
EUR 150
Description: Fluorescence-probe-of-mitochondrial-membrane-potential

Embedding Mold, Polyethylene, Light Microscopy - 12mm x 8mm

23184-1 1pkg
EUR 113

Embedding Molds, Polyethylene, Light Microscopy - 16mm x 8mm

23185-1 1pkg
EUR 113

Acryloxyethyl thiocarbamoyl Rhodamine B

25404-100 100mg
EUR 306
Description: 1821380-54-4

PEGylated Liposomal Rhodamine B Dye

PHC001RB 1 ml
EUR 140.62
Description: This product includes 1 ml of 0.1 mM Rhodamine B encapsulated in liposome composed of HSPC:DSPE-PEG2000:Cholesterol (w/w 3:1:1).

Rhodamine B (RDB) Polyclonal Antibody

CAU31903-100ul 100ul
EUR 267.8

Rhodamine B (RDB) Polyclonal Antibody

CAU31903-200ul 200ul
EUR 334.3

Rhodamine B, hexyl ester, perchlorate

22211-10mg 10 mg
EUR 86
Description: Positively charged rhodamine dyes (such as rhodamine esters and rosamines) are selectively localized in mitochondria, thus they are widely used for labeling mitochondria of live cells.

Embedding Molds, Polyethylene, Light Microscopy - 19mm x 13mm

23186-1 1pkg
EUR 92

RBITC [Rhodamine B 5-isothiocyanate]

TD0059-10mg 10mg Ask for price
Description: RBITC [Rhodamine B 5-isothiocyanate]

RBITC [Rhodamine B 5-isothiocyanate]

TD0059-1g 1g Ask for price
Description: RBITC [Rhodamine B 5-isothiocyanate]

RBITC [Rhodamine B 5-isothiocyanate]

TD0059-1mg 1mg Ask for price
Description: RBITC [Rhodamine B 5-isothiocyanate]

RBITC [Rhodamine B 5-isothiocyanate]

TD0059-50mg 50mg Ask for price
Description: RBITC [Rhodamine B 5-isothiocyanate]

RBITC [Rhodamine B 5-isothiocyanate]

TD0059-5mg 5mg Ask for price
Description: RBITC [Rhodamine B 5-isothiocyanate]

Embedding Stubs, Polyethylene, Light Microscopy - 4mm thin - white

23189-1 1pkg
EUR 210

Methacryloxyethyl thiocarbamoyl rhodamine B

23591-1 1g
EUR 2827

Methacryloxyethyl thiocarbamoyl rhodamine B

23591-100 100mg
EUR 328

Rhodamine 6G

HY-D0309 10mM/1mL
EUR 151.2

Rhodamine 6G

561580 25.0g
EUR 170

Rhodamine 6G

T16744-10mg 10mg Ask for price
Description: Rhodamine 6G

Rhodamine 6G

T16744-1g 1g Ask for price
Description: Rhodamine 6G

Rhodamine 6G

T16744-1mg 1mg Ask for price
Description: Rhodamine 6G

Rhodamine 6G

T16744-50mg 50mg Ask for price
Description: Rhodamine 6G

Rhodamine 6G

T16744-5mg 5mg Ask for price
Description: Rhodamine 6G

RBITC-[Rhodamine-B-5-isothiocyanate]

E37F-418 100-mg
EUR 253.33
Description: Peptide,-protein,-antibody-labeling-reagents.-Thiol-group-was-labeled-with-fluorescent-reagent.-Fluorescent-reagent-containing-maleic-anhydride-group.

Lissamine rhodamine B sulfonyl chloride

598997 1.0g
EUR 550

Rhodamine B octadecyl ester perchlorate

GW0490-100 100
EUR 845.7

Rhodamine B octadecyl ester perchlorate

GW0490-20 20
EUR 232

Lissamine rhodamine B sulfonyl chloride

T20773-10mg 10mg Ask for price
Description: Lissamine rhodamine B sulfonyl chloride

Lissamine rhodamine B sulfonyl chloride

T20773-1g 1g Ask for price
Description: Lissamine rhodamine B sulfonyl chloride

Lissamine rhodamine B sulfonyl chloride

T20773-1mg 1mg Ask for price
Description: Lissamine rhodamine B sulfonyl chloride

Lissamine rhodamine B sulfonyl chloride

T20773-50mg 50mg Ask for price
Description: Lissamine rhodamine B sulfonyl chloride

Lissamine rhodamine B sulfonyl chloride

T20773-5mg 5mg Ask for price
Description: Lissamine rhodamine B sulfonyl chloride

Rhodamine B isothiocyanate (mixed isomers)

T37607-10mg 10mg Ask for price
Description: Rhodamine B isothiocyanate (mixed isomers)

Rhodamine B isothiocyanate (mixed isomers)

T37607-1g 1g Ask for price
Description: Rhodamine B isothiocyanate (mixed isomers)

Rhodamine B isothiocyanate (mixed isomers)

T37607-1mg 1mg Ask for price
Description: Rhodamine B isothiocyanate (mixed isomers)

Rhodamine B isothiocyanate (mixed isomers)

T37607-50mg 50mg Ask for price
Description: Rhodamine B isothiocyanate (mixed isomers)

Rhodamine B isothiocyanate (mixed isomers)

T37607-5mg 5mg Ask for price
Description: Rhodamine B isothiocyanate (mixed isomers)

Rhodamine 6GX

30111-62 25G
EUR 43.05

Rhodamine 6G (C.I. 45160)

GT5023-100G 100 g
EUR 108

Rhodamine 6G (C.I. 45160)

GT5023-25G 25 g
EUR 58.8

Rhodamine 6G (C.I. 45160)

GT5023-5G 5 g
EUR 49.2

Rhodamine 6G (C.I. 45160)

GT5023-100 100
EUR 59.3

Rhodamine 6G (C.I. 45160)

GT5023-25 25
EUR 19

Rhodamine 6G (C.I. 45160)

GT5023-5 5
EUR 12.1

Rhodamine 6G, C.I. 45160

25004-25 25g Ask for price
Description: 989-38-8

Rhodamine 6G, C.I. 45160

25004-50 50g Ask for price
Description: 989-38-8

Rhodamine B solution, 0.2% in isopropanol

GT1789-100ML 100 ml
EUR 60

Rhodamine B solution, 0.2% in isopropanol

GT1789-500ML 500 ml
EUR 103.2

Rhodamine B solution, 0.2% in isopropanol

GT1789-100 100
EUR 19.8

Rhodamine B solution, 0.2% in isopropanol

GT1789-500 500
EUR 55.4

Polyclonal Antibody to Rhodamine B (RDB)

PAO062Ge01 100ul
EUR 279

Rhodamine-DHPE

60026-1 EA
EUR 252

Rhodamine 123 (chloride)

C3140-10 10 mg
EUR 134.4
Description: Rhodamine 123 is a fluorescent tracer dye used as substrate for P-glycoprotein.Fluorescent tracer dyes represent a class of sub-cellular probes allowing the examination of cellular processes in real-time with minimal impact upon such processes.

Rhodamine 123 (chloride)

C3140-100 100 mg
EUR 477.6
Description: Rhodamine 123 is a fluorescent tracer dye used as substrate for P-glycoprotein.Fluorescent tracer dyes represent a class of sub-cellular probes allowing the examination of cellular processes in real-time with minimal impact upon such processes.

Rhodamine 123 (chloride)

C3140-25 25 mg
EUR 195.6
Description: Rhodamine 123 is a fluorescent tracer dye used as substrate for P-glycoprotein.Fluorescent tracer dyes represent a class of sub-cellular probes allowing the examination of cellular processes in real-time with minimal impact upon such processes.

Rhodamine 110 chloride

341324 250.0mg
EUR 350

Rhodamine-12-dUTP

NU-803-RHOX-L 5 x 10µl
EUR 281.5
Description: Rho-green-X-dUTP, RhG-dUTP, R110-dUTP

Rhodamine-12-dUTP

NU-803-RHOX-S 10µl
EUR 99.4
Description: Rho-green-X-dUTP, RhG-dUTP, R110-dUTP

Rhodamine-12-dCTP

NU-809-RHOX-L 5 x 10µl
EUR 319.3
Description: Rho-green-X-dCTP, RhG-dCTP, R110-dCTP

The imply age of the members was 36.41 ± 6.379. Male getting old with out impression of confounders was correlated with a lower in sperm focus and motility and an elevated in sperm necrosis. Sperm focus and progressive motility had been negatively associated to the presence of confounders as wine consumption (sperm motility), urogenital an infection (sperm focus and motility), varicocele (sperm focus) and consanguinity (sperm motility). Urogenital an infection, varicocele and consanguinity positively correlated with sperm necrosis. The most necessary discovering was the statement of a unfavourable impact of male getting old on sperm parameters resembling focus, motility, and viability.

It is feasible to hypothesize age-dependent adjustments of testicular setting, in all probability associated to reactive oxygen species manufacturing. The demonstration, in a big pattern of sufferers, that getting old influences sperm high quality strongly motivates additional analysis centered on the mechanisms concerned in this phenomenon and its results on offspring health. We talk about challenges and alternatives to combine this method with multibeam scanning electron microscopy workflows in addition to a number of imaging modalities for mapping of organ and tissue construction and perform. Semen evaluation was carried out by mild microscopy and transmission electron microscopy mathematically elaborated.