Non-invasive Quantification of Cell Wall Porosity by Fluorescence Quenching Microscopy

Non-invasive Quantification of Cell Wall Porosity by Fluorescence Quenching Microscopy

All micro organism, fungi and plant cells are surrounded by a cell wall. This complicated community of polysaccharides and glycoproteins gives mechanical assist, defines cell form, controls cell development and influences the trade of substances between the cell and its environment. Despite its identify, the cell wall is a versatile, dynamic construction. However, because of the lack of non-invasive strategies to probe the construction, comparatively little is understood concerning the synthesis and dynamic transforming of cell partitions. Here, we describe a non-invasive technique that quantifies a key physiological parameter of cell partitions, the porosity, i.e., the dimensions of areas between cell wall elements.

This technique measures the porosity-dependent lower of the plasma membrane-localized fluorescent dye FM4-64 within the presence of the extracellular quencher Trypan blue. This technique is utilized to micro organism, fungi and plant cell partitions to detect dynamic modifications of porosity in response to environmental cues.The most vital virulence issue within the Cryptococcus genus is the polysaccharide capsule. This genus contains a number of species that trigger life-threatening invasive illness. An enhance in capsule thickness is vital throughout fungal an infection.

The capsule is normally imaged utilizing India ink, and essential insights on the dynamics of its development have been obtained utilizing capsule-binding proteins reminiscent of particular antibodies or complement. We have developed another technique that permits each static and time-lapse imaging of the capsule utilizing Percoll®, a suspension of nanometric spheres that don’t penetrate the capsule. Given that these particles have a better refractive index than the capsule, the latter could be imaged by differential interference distinction (DIC) microscopy. Static commentary of the capsule with DIC and Percoll® ends in capsule thickness measurements that match these made with India ink.

Using capsule-inducing media, a glass-bottom incubation chamber and a live-imaging system outfitted for DIC microscopy, this technique permits time-lapse imaging of capsule development. In distinction with India ink staining, DIC imaging of Percoll® exclusion halos lead to crisp photos. The best benefit of this technique, although, is that in contrast to India ink, the Percoll® particles are non-toxic and in contrast to opsonins they don’t bind the capsule, leading to observations of capsule development which are free from interference of sure proteins on capsule physiology.

microscopy-based strategy for figuring out development likelihood and lag time of particular person bacterial cells

The growth of related predictive fashions for single-cell lag time and development likelihood close to development limits is of important significance for predicting pathogen habits in meals. The classical strategies for information acquisition on this area are based mostly on turbidity measurements of tradition media in microplate wells inoculated with roughly one bacterial cell per effectively. Yet, these strategies are labour intensive and would profit from larger throughput. In this research, we developed a quantitative experimental technique utilizing automated microscopy to find out the single-cell development likelihood and lag time.
The developed technique consists of the use of direct cell commentary with phase-contrast microscopy outfitted with a 100× goal and a high-resolution gadget digicam. The technique isn’t a time-lapse technique however is predicated on the commentary of excessive numbers of colonies for a given time. Automation of picture acquisition and picture evaluation was used to achieve a excessive throughput. The single-cell development chances and lag occasions of 4 strains of Listeria monocytogenes have been decided at 4 °C. The microscopic technique was proven to be a promising technique for the dedication of particular person lag occasions and development likelihood on the single-cell stage.
We exhibit that beneath ambient and humidity-controlled situations, operation of bimodal excitation single-scan electrical drive microscopy with no electrical suggestions loop will increase the spatial decision of floor electrical property measurements all the way down to the 5 nm restrict. This technical enchancment is featured on epitaxial graphene layers on SiC, which is used as a mannequin pattern. The experimental situations developed to attain such decision are mentioned and linked to the secure imaging achieved utilizing the proposed technique.
The software of the herein reported technique is achieved with out the necessity to apply DC bias voltages, which advantages specimens which are extremely delicate to polarization. Besides, it permits the simultaneous parallel acquisition of floor electrical properties (reminiscent of contact potential distinction) on the similar scanning price as in amplitude modulation atomic drive microscopy (AFM) topography measurements. This makes it engaging for purposes in excessive scanning velocity AFM experiments in numerous fields for materials screening and metrology of semiconductor methods.
Non-invasive Quantification of Cell Wall Porosity by Fluorescence Quenching Microscopy

A bidirectional scanning technique for scanning transmission X-ray microscopy

Scanning mode is a key issue for the excellent efficiency, together with imaging effectivity, of scanning transmission X-ray microscopy (STXM). Herein is introduced a bidirectional scanning technique designed for STXM with an S-shaped transferring observe. In this technique, artificially designed ramp waves are generated by a piezo-stage controller to regulate the two-dimensional scanning of the pattern. The pattern place info is measured utilizing laser interferometric sensors and despatched to a field-programmable gate array (FPGA) board which additionally acquires the X-ray indicators concurrently from the detector.

Fluorescence Microscopy Aid

4400 3 ml
EUR 216.6
Description: This is Counter - Stain/Blocking Diluent with optimal formula for preparaiton of final dilutions of FITC conjugate prior to use in FA procedures.

Exosome electron microscopy imaging (EM)

S109 -
EUR 600

Embedding Stubs, Polyethylene, Light Microscopy - 10mm thick

23197-1 1pkg
EUR 375

Embedding Mold, Polyethylene, Light Microscopy - 12mm x 8mm

23184-1 1pkg
EUR 113

Embedding Molds, Polyethylene, Light Microscopy - 16mm x 8mm

23185-1 1pkg
EUR 113

Embedding Molds, Polyethylene, Light Microscopy - 19mm x 13mm

23186-1 1pkg
EUR 92

Embedding Stubs, Polyethylene, Light Microscopy - 4mm thin - white

23189-1 1pkg
EUR 210

EZClick? Stearoylated Protein Assay Kit (FACS/Microscopy), Red Fluorescence

K418-100 each
EUR 588

EZClick? Stearoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence

K453-100 each
EUR 588

EZClick? Palmitoylated Protein Assay Kit (FACS/Microscopy), Red Fluorescence

K416-100 each
EUR 588

EZClick? Global RNA Synthesis Assay Kit (FACS/Microscopy), Red Fluorescence

K718-100 each
EUR 639.6

EZClick? Global RNA Synthesis Assay Kit (FACS/Microscopy), Red Fluorescence

K462-100 each
EUR 639.6

EZClick? Palmitoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence

K452-100 each
EUR 588

EZClick? Myristoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence

K497-100 each
EUR 588

EZClick? Global Protein Synthesis Assay Kit (FACS/Microscopy), Red Fluorescence

K715-100 each
EUR 660

EZClick? Global Protein Synthesis Assay Kit (FACS/Microscopy), Green Fluorescence

K459-100 each
EUR 660

EZClick? Global Phospholipid Synthesis Assay Kit (FACS/Microscopy), Red Fluorescence

K717-100 each
EUR 614.4

EZClick? EdU Cell Proliferation/DNA Synthesis Kit (FACS/Microscopy), Red Fluorescence

K946-100 each
EUR 639.6

EZClick? O-GalNAc Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)

K560-100 each
EUR 588

EZClick? O-GlcNAc Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)

K714-100 each
EUR 614.4

Orcein

GT0012-10G 10 g
EUR 151.2

Orcein

GT0012-1G 1 g
EUR 64.8

Orcein

GT0012-25G 25 g
EUR 246

Orcein

GT0012-5G 5 g
EUR 103.2

Orcein

T19835-10mg 10mg Ask for price
Description: Orcein

Orcein

T19835-1g 1g Ask for price
Description: Orcein

Orcein

T19835-1mg 1mg Ask for price
Description: Orcein

Orcein

T19835-50mg 50mg Ask for price
Description: Orcein

Orcein

T19835-5mg 5mg Ask for price
Description: Orcein

Orcein

GT0012-1 1
EUR 23.8

Orcein

GT0012-10 10
EUR 95

Orcein

GT0012-25 25
EUR 174

Orcein

GT0012-5 5
EUR 55.4

EZClickTM Fucose (FucAz) Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)

K592-100 each
EUR 588

EZClick? Sialic Acid (ManAz) Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)

K441-100 each
EUR 588

Differentiating Solution (For Orcein Stain)

DSA125 125 ml
EUR 85.2

Differentiating Solution (For Orcein Stain)

DSA500 500 ml
EUR 146.4

Aceto Orcein

40140045-1 1 g
EUR 25.45

Aceto Orcein

40140045-2 10 g
EUR 86.86

Aceto Orcein

40140045-3 25 g
EUR 232.33

Orcein, Natural

O03040 1G
EUR 109.75

Orcein Shikata

RRSP117-D 500ml
EUR 65.24

Orcein Shikata

RRSP117-E 1L
EUR 115.39

Orcein Shikata

RRSP117-F 2.5L
EUR 252.58

Orcein Solution

OAA125 125 ml
EUR 79.2

Orcein Solution

OAA500 500 ml
EUR 130.8

Orcein Solution

OAA999 1000 ml
EUR 177.6

Orcein, Synthetic

O03048 1G
EUR 164.78

Aceto Orcein 2%

40140046-1 100 mL
EUR 34.74

Orcein Stain Kit

K1432-125 each
EUR 601.2

Orcein Stain Kit

K1432-30 each
EUR 430.8

Orcein Acetic (La Cour)

RRSP118-D 500ml
EUR 56.66

Orcein Acetic (La Cour)

RRSP118-E 1L
EUR 102.23

Orcein Stain Kit (For Hepatitis B and Elastic Fibers)

HBK-1 1 kit(s)
EUR 286.8

Orcein Stain Kit (For Hepatitis B and Elastic Fibers)

HBK-2 1 kit(s)
EUR 172.8

Cardboard tray for 20 microscope slides

DD65205 EACH
EUR 19.2

Box for 25 microscope slides purple colour

DD49719 EACH
EUR 19.2

Box for 25 microscope slides orange colour

DD49720 EACH
EUR 19.2

NovaUltra Orcein Elastin Stain Kit

IW-3018 -
EUR 225

Plastic box for 100 microscope slides (red colour)

DD37503 EACH
EUR 38.4

Plastic box for 100 microscope slides (blue colour)

DD37501 EACH
EUR 38.4

Box for 25 microscope slides turquoise colour

DD49721 EACH
EUR 19.2

Plastic box for 50 microscope slides (blue colour)

DD37507 EACH
EUR 32.4

Polystyrene box for 100 microscope slides blue colour

DD139537 EACH
EUR 22.8

Polystyrene box for 100 microscope slides green colour

DD139538 EACH
EUR 22.8

C-Mount for All SLS Lab Basics Max III Microscopes

SLS2300 EACH
EUR 127.2

25-place microscope slide box (for 75 x 25 mm slides)

PO202 each
EUR 73.2

100-place microscope slide box (for 75 x 25 mm slides)

PO201 each
EUR 84

100-place microscope slide box (for 75 x 50 mm slides)

PO203 each
EUR 152.4

MiTeGen Kit V201 Stereo Microscope kit for MicroCrystallography:

ZV20-MTGKV201 1 KIT
EUR 16532
Description: MiTeGen Kit V201 Stereo Microscope kit for MicroCrystallography:- Ergonomic adjustable eyepieces- A wide array of accessories available- 7.5 to 150 x magnification range- ZEISS SteREO Discovery.V20- ZEISS SteREO Discovery.V20 body,- Human Interface Panel HIP,- Dust Protection Set M ,- Binoc Phototube Ergo Stereo 5-45 ,- Eyepiece PL 10x/23 Br foc ,- Folding Eyecup ,- Stand base Profile S,- Transmitted Light Equipment S- Cold-light source Zeiss CL6000 LED- 6200K color temperature,- control of intensity & 6 memory positions,- filter slider for 2 filters 35x26x4mm (sold separately)- Analyzer S Rotatable,- Polarizer D =84mm,- Coarse/fine drive w/column S 490mm- Mount S with 76 mm Diameter Support- Achromat S 1.0x Reo WD=63 lens

Plastic box for 100 microscope slides (assorted colours) cork bottom

DD10197 PK5
EUR 87.6

C-Mount for All SLS Lab Basics Magnum and Magtex Microscopes

SLS2302 EACH
EUR 145.2

MiTeGen Kit V81 Stereo Microscope kit for Crystallography:

ZV20-MTGKV81 1 KIT
EUR 13998
Description: MiTeGen Kit V81 Stereo Microscope kit for Crystallography:- ZEISS Stereo Discovery V8 Microscope:- ZEISS Stereo Discovery.V8 body,- Dust Protection Set M ,- Binocular Phototube Ergo Stereo 5-45 ,- Eyepiece PL 10x/23 Br foc ,- Folding Eyecup ,- Stand base Profile S,- Transmitted Light Equipment S:- Cold-light source ZEISS CL6000 LED- 6200K color temperature, control of intensity & 6 memory positions,- filter slider for 2 filters (35 x 26 x 4mm, (filters sold separately)- Analyzer S Rotatable,- Polarizer D =84mm,- Manual focus drive fa/ Discovery,- Mount S with 76 mm Diameter Support- Achromatic S 1.0x Reo WD=63 lens

IncuView Integrated Microscop Shelf, 45L (For H3565-45 Only)

H3565-45-LCI 1 each
EUR 1884.6

MiTeGen Kit V121 Stereo Microscope kit for Crystallography:

ZV20-MTGKV121 1 KIT
EUR 15282
Description: MiTeGen Kit V121 Stereo Microscope kit for Crystallography:- ZEISS Stereo Discovery V12 Microscope:- ZEISS Stereo Discovery.V8 body,- Dust Protection Set M ,- Binocular Phototube Ergo Stereo 5-45 ,- Eyepiece PL 10x/23 Br foc ,- Folding Eyecup ,- Stand base Profile S,- Transmitted Light Equipment S:- Cold-light source ZEISS CL6000 LED- 6200K color temperature,- control of intensity & 6 memory positions,- filter slider for 2 filters (35 x 26 x 4mm, (filters sold separately)- Analyzer S Rotatable,- Polarizer D =84mm,- Manual focus drive fa/ Discovery,- Mount S with 76 mm Diameter Support- Achromatic S 1.0x Reo WD=63 lens

Microscope slide dispenser for box of 72 standard 25 x 75mm slides

M7100-SD 1/pack
EUR 83.89
Description: Microscope slide dispenser

MiTeGen Kit Stemi 305 Stereo Microscope kit for Crystallography:

ZV20-MTGK305 1 KIT
EUR 2589
Description: MiTeGen Kit Stemi 305 Stereo Microscope kit for Crystallography:Stemi 305 trinocular body w/ integrated camera portIncludes adapter for C-mount lensesStand K Edu w/ integrated transmissive LED cold light and polarizerSpot illuminator w/ polarizerAnalyzer (rotatable)

MiTeGen Crystallography Microscope Kit for Protein Crystallography:

ZV20-MTGK508-PC1 1 KIT
EUR 6492
Description: MiTeGen Crystallography Microscope Kit for Protein Crystallography:- Stemi 508 Stand M- Magnification: 10-80x (with 16x eyepieces)- Free Working Distance: 92 mm- Eyepieces (2 x 10x & 2 x 16x)- Cold light (LED) transillumination source with large working area stand- Rotatable and slidable mirror for brightfield, darkfield and oblique transillumination- Plain mirror side for crisp, diffuse mirror side for homogeneous illumination- Trinocular Camera Port Configuration- Camera Adapter 60N- Viewing angle 35 degrees with adjustable interocular distance- Camera Port- Analyzer (rotatable)- Transillumination Polarizer- Dual flex-neck top-side LED illumination

IncuView Integrated Microscop Shelf, 180L (For H3565-180, 180HD and 180HDO2 Only)

H3565-180-LCI 1 each
EUR 1980.9

Nanosoft Clipping Station for the clipping of cryoEM grids for Thermo Fisher autoloader-based microscopes.

M-CEM-NS-CLSTN 1 UNIT
EUR 2400
Description: Nanosoft Clipping Station for the clipping of cryoEM grids for Thermo Fisher autoloader-based microscopes.

Nanosoft Clipping Station for the clipping of cryoEM grids for Thermo Fisher autoloader-based microscopes.

M-CEM-NS-CLSTN01 each
EUR 2400
Description: Nanosoft Clipping Station for the clipping of cryoEM grids for Thermo Fisher autoloader-based microscopes.

Microscope Pack 1 CX23 100x Oil

MIC1968 EACH
EUR 1827.42

Microscope Pack 2 CX23 BIN LED

MIC1970 EACH
EUR 1658.11

FlyStuff Microscope

59-502 1 Microscope/Unit
EUR 1801.67
Description: Binocular, w/base

Microscope Slides, Micropure

22245-20 1box
EUR 380

Microscope Slides, Micropure

22245-1 1box
EUR 32

Microscope Slide Box 25 ULT safe

MIC4092 EACH
EUR 19.37

Microscope Slides, Plain

07441-1 1box
EUR 50

Microscope Cover Slips No. 1.5 16mm

MIC2182 PK100
EUR 10.8

Microscope Cover Slips No. 0 19mm dia

MIC2180 PK100
EUR 12

Motic SMZ-168-BL Microscope

MIC2800 EACH
EUR 1357.74

Microscope Slide Box 100 Place Tall

MIC4080 EACH
EUR 42

Microscope Slide Box True North

MIC4090 EACH
EUR 47.52

Quartz Microscope Slide, 1"x2" (25.4 x 50.8 mm) x 0.5 mm Thin

24962-1 1EA
EUR 217

Quartz Microscope Slide, 1"x3" (25.4 x 76.2 mm) x 0.5 mm Thin

24963-1 1EA
EUR 192

BRAND(TM) MICROSCOPE SLIDE CUT EDGES

BR474701-2500EA PK2500
EUR 218.4

Vinyl microscope slides RINZL

DD52951 PK144
EUR 24.62

Microscope Slide Holder Block

24764-1 1each
EUR 148

Quartz Microscope Slide, 1"x1" (25.4 x 25.4 mm) x 1 mm Thick

24957-1 1EA
EUR 58

Microscope Cover Slips No. 0 18x18mm

MIC2158 PK100
EUR 3.6

Microscope Cover Slips No. 0 22x22mm

MIC2160 PK100
EUR 3.6

Microscope Cover Slips No. 1 18x18mm

MIC2162 PK100
EUR 2.4

Microscope Cover Slips No. 1 22x22mm

MIC2164 PK100
EUR 3.6

Microscope Cover Slips No. 1.5 18x18mm

MIC2166 PK100
EUR 2.4

Microscope Cover Slips No. 1.5 22x22mm

MIC2168 PK100
EUR 3.6

Microscope Cover Slips No.1.5 22x26mm

MIC2170 PK100
EUR 4.8

Microscope Cover Slips No. 1.5 22x32mm

MIC2172 PK100
EUR 4.8

Microscope Cover Slips No. 1.5 22x40mm

MIC2174 PK100
EUR 6

Microscope Cover Slips No. 2 18x18mm

MIC2176 PK100
EUR 3.6

Microscope Cover Slips No. 2 22x22mm

MIC2178 PK100
EUR 4.8

Microscope Slide Mailing Tubes

23998-10 10pkg
EUR 40

VIKEM(R) VINYL MICROSCOPE COVER EXTRA

BAF243040000-1EA EACH
EUR 32.88

Stereo Microscope Trinocular

MIC1590 EACH
EUR 1274.27

Microscope Slide Boxes,100-Place, Red

LC7004-001 Ea
EUR 84

Microscope Slide Boxes,100-Place, Red

LC7004-010 10/PK
EUR 181.2

Microscope Slide Boxes,100-Place, Red

LC7004-100 100/CS
EUR 967.2

Microscope Slide Boxes,25-Place, Red

LC7009-001 EA
EUR 79.2

Microscope Slide Boxes,25-Place, Red

LC7009-010 10/PK
EUR 148.8

Microscope Slide Boxes,25-Place, Red

LC7009-100 100/CS
EUR 684

MIGHTY SCOPE(TM) DIGITAL MICROSCOPE 5M

Z742668-1EA EACH
EUR 451.74

Super Frosted Microscope Slides

21912-1 1box
EUR 60

Digi-Pad Microscope Tablet Camera

MIC0115 EACH
EUR 1807.2

Microscope Slides Plain 1.2-1.5mm 76x26mm

MIC2154 PK50
EUR 4.08

Microscope Slides Plain 1.0mm 76x52mm

MIC2156 PK50
EUR 19.2

Microscope Slide Boxes,100-Place, Blue

LC7002-001 Ea
EUR 84

Microscope Slide Boxes,100-Place, Blue

LC7002-010 10/PK
EUR 181.2

Microscope Slide Boxes,100-Place, Blue

LC7002-100 100/CS
EUR 967.2

Microscope Slide Boxes,25-Place, Blue

LC7007-001 EA
EUR 79.2

Microscope Slide Boxes,25-Place, Blue

LC7007-010 10/PK
EUR 148.8

Microscope Slide Boxes,25-Place, Blue

LC7007-100 100/CS
EUR 684

Microscope Slide Storage Cabinet

22352-1 1each
EUR 278

Microscope Slide Holder, Peel-A-Way

19801A-1 1Box
EUR 56

Motic BA210 LED Microscope Binocular

MIC2004 EACH
EUR 1667.25

Motic BA310 LED Microscope Binocular

MIC2016 EACH
EUR 2250.85

BA310 Elite Trinocular microscope

MIC2937 EACH
EUR 2667.34

BA210E Elite Binocular Microscope

MIC5633 EACH
EUR 1809.56

Microscope Slide Boxes,100-Place, Green

LC7003-001 Ea
EUR 84

Microscope Slide Boxes,100-Place, Green

LC7003-010 10/PK
EUR 181.2

Microscope Slide Boxes,100-Place, Green

LC7003-100 100/CS
EUR 967.2

Microscope Slide Boxes,100-Place, White

LC7006-001 Ea
EUR 84

Microscope Slide Boxes,100-Place, White

LC7006-010 10/PK
EUR 181.2

Microscope Slide Boxes,100-Place, White

LC7006-100 100/CS
EUR 967.2

Microscope Slide Boxes,25-Place, Green

LC7008-001 EA
EUR 79.2

Microscope Slide Boxes,25-Place, Green

LC7008-010 10/PK
EUR 148.8

Microscope Slide Boxes,25-Place, Green

LC7008-100 100/CS
EUR 684

Microscope Slide Boxes,25-Place, White

LC7011-001 EA
EUR 79.2

Microscope Slide Boxes,25-Place, White

LC7011-010 10/PK
EUR 148.8

Microscope Slide Boxes,25-Place, White

LC7011-100 100/CS
EUR 684

Superfrost® Plus microscope slides

PO103 box (72 slides)
EUR 123.6

Polysine Coated Microscope Slides

26414-1 1box
EUR 82

Polysine Coated Microscope Slides

26414-20 20box
EUR 585

FlyStuff Trinocular Microscope

59-503 1 Microscope/Unit
EUR 2779.21
Description: Trinocular, w/base

Motic BA310 LED Microscope Trinocular

MIC2018 EACH
EUR 2545.92

SLS Lab Basics Star-24 LED Stereo Microscope

MIC2026 EACH
EUR 1422

Since the info recorded by the FPGA include the actual place of every scanned level, the affect of the backlash brought on by the back-turning motion on the STXM picture could be eradicated. By using an tailored post-processing program, a re-meshed high-resolution STXM picture could be obtained. This S-track bidirectional scanning technique in fly-scan mode has been carried out on the STXM endstation on the Shanghai Synchrotron Radiation Facility (SSRF), and efficiently resolved the ∼30 nm interval between the innermost strips of a Siemens star. This work removes the limitation on bidirectional scanning brought on by motor backlash and vibration, and considerably improves the effectivity of STXM experiments.